| Title: | Methyl jasmonate-induced emission of biogenic volatiles is biphasic in cucumber: a high-resolution analysis of dose dependence |
| Author(s): | Jiang Y; Ye J; Li S; Niinemets U; |
| Address: | "Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, Tartu 51014, Estonia. College of Art, Changzhou University, Gehu 1, Changzhou 213164, Jiangsu, China. Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking) |
| Abstract: | "Methyl jasmonate (MeJA) is a key airborne elicitor activating jasmonate-dependent signaling pathways, including induction of stress-related volatile emissions, but how the magnitude and timing of these emissions scale with MeJA dose is not known. Treatments with exogenous MeJA concentrations ranging from mild (0.2 mM) to lethal (50 mM) were used to investigate quantitative relationships among MeJA dose and the kinetics and magnitude of volatile release in Cucumis sativus by combining high-resolution measurements with a proton-transfer reaction time-of-flight mass spectrometer (PTR-TOF-MS) and GC-MS. The results highlighted biphasic kinetics of elicitation of volatiles. The early phase, peaking in 0.1-1 h after the MeJA treatment, was characterized by emissions of lipoxygenase (LOX) pathway volatiles and methanol. In the subsequent phase, starting in 6-12 h and reaching a maximum in 15-25 h after the treatment, secondary emissions of LOX compounds as well as emissions of monoterpenes and sesquiterpenes were elicited. For both phases, the maximum emission rates and total integrated emissions increased with applied MeJA concentration. Furthermore, the rates of induction and decay, and the duration of emission bursts were positively, and the timing of emission maxima were negatively associated with MeJA dose for LOX compounds and terpenoids, except for the duration of the first LOX burst. These results demonstrate major effects of MeJA dose on the kinetics and magnitude of volatile response, underscoring the importance of biotic stress severity in deciphering the downstream events of biological impacts" |
| Keywords: | "Acetates/*administration & dosage/*metabolism/pharmacology Cucumis sativus/drug effects/*metabolism Cyclopentanes/*administration & dosage/*metabolism/pharmacology Dose-Response Relationship, Drug Gas Chromatography-Mass Spectrometry Monoterpenes/metaboli;" |
| Notes: | "MedlineJiang, Yifan Ye, Jiayan Li, Shuai Niinemets, Ulo eng 322603/ERC_/European Research Council/International Research Support, Non-U.S. Gov't England 2017/10/06 J Exp Bot. 2017 Jul 20; 68(16):4679-4694. doi: 10.1093/jxb/erx244" |
