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J Microbiol

Title:		Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression
Author(s):		Hwang J; Jung D; Kim J;
Address:		"Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea. jmkim@cnu.ac.kr"
Journal Title:		J Microbiol
Year:		2022
Volume:		20220714
Issue:		8
Page Number:		843 - 848
DOI:		10.1007/s12275-022-2213-x
ISSN/ISBN:		1976-3794 (Electronic) 1225-8873 (Linking)
Abstract:		"Decapping of mRNA is a key regulatory step for mRNA decay and translation. The RNA helicase, Dhh1, is known as a decapping activator and translation repressor in yeast Saccharomyces cerevisiae. Dhh1 also functions as a gene-specific positive regulator in the expression of Ste12, a mating-specific transcription factor. A previous study showed that the N-erminal phosphorylation of Dhh1 regulates its association with the mRNA-binding protein, Puf6, to affect the protein translation of Ste12. Here, we investigated the roles of the phosphorylated residues of Dhh1 in yeast mating process and Ste12 expression. The phospho-deficient mutation, DHH1-T10A, was associated with decreased diploid formation during mating and decreased level of the Ste12 protein in response to alpha-mating pheromone. A kinase overexpression analysis revealed that Ste12 protein expression was affected by overexpression of Fus3 MAP kinase or Tpk2 kinase. Tpk2 was shown to be responsible for phosphorylation of Dhh1 at Thr10. Our study shows that overexpression of Fus3 or Tpk2 alters the Dhh1-Puf6 protein interaction and thereby affects Ste12 protein expression"
Keywords:		"Cyclic AMP-Dependent Protein Kinases/genetics/metabolism DEAD-box RNA Helicases/genetics/metabolism Fungal Proteins/genetics Gene Expression Regulation, Fungal Mating Factor/genetics/metabolism Mitogen-Activated Protein Kinases/genetics/metabolism Phospho;"
Notes:		"MedlineHwang, Jaehee Jung, Daehee Kim, Jinmi eng Korea (South) 2022/07/15 J Microbiol. 2022 Aug; 60(8):843-848. doi: 10.1007/s12275-022-2213-x. Epub 2022 Jul 14"