Title: | Localization of the FAR3 gene: genetic mapping and molecular cloning using a chromosome walk-'n'-roll strategy |
Address: | "Institute of Molecular Biology, University of Oregon, Eugene 97403, USA" |
ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
Abstract: | "FAR3 is a newly-discovered yeast gene required specifically for pheromone-mediated cell cycle arrest. I have used strains harboring the far3-1 mutation to map the gene to the right arm of chromosome XIII, establishing the gene order CEN13-LYS7-MCM1-FAR3. I cloned the FAR3 gene based on its genetic map position using a strategy that combined chromosome walking and a related technique termed 'chromosome rolling'. In addition to the genetic and physical localization of FAR3, I present data that suggest corrections to the tentative map positions of VAN1 and ARG80" |
Keywords: | "Cell Cycle/*genetics Cell Cycle Proteins/*genetics *Chromosome Mapping Chromosome Walking Chromosomes, Fungal Cloning, Molecular Crosses, Genetic Fungal Proteins/*genetics *Genes, Fungal Restriction Mapping Saccharomyces cerevisiae/*genetics *Saccharomyce;" |
Notes: | "MedlineHorecka, J eng GM07759/GM/NIGMS NIH HHS/ GM30027/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. England 1995/06/15 Yeast. 1995 Jun 15; 11(7):691-6. doi: 10.1002/yea.320110710" |