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Talanta

Title:		Comprehensive two-dimensional gas chromatography combined to multivariate data analysis for detection of disease-resistant clones of Eucalyptus
Author(s):		Hantao LW; Toledo BR; Ribeiro FA; Pizetta M; Pierozzi CG; Furtado EL; Augusto F;
Address:		"Institute of Chemistry, University of Campinas (Unicamp) and Instituto Nacional de Ciencia e Tecnologia em Bioanalitica (INCTBio), IQ, Unicamp, CP 6154, 13084-971 Campinas, Sao Paulo, Brazil"
Journal Title:		Talanta
Year:		2013
Volume:		20130826
Issue:
Page Number:		1079 - 1084
DOI:		10.1016/j.talanta.2013.08.033
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"In this paper it is reported the use of the chromatographic profiles from volatile fractions of plant clones - in this case, hybrids of Eucalyptus grandisxEucalyptus urophylla - to determine specimens susceptible to rust disease. The analytes were isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography combined to fast quadrupole mass spectrometry (GCxGC-qMS). Parallel Factor Analysis (PARAFAC) was employed for estimate the correlation between the chromatographic profiles and resistance against Eucalyptus rust, after preliminary variable selection performed by Fisher ratio analysis. The proposed method allowed the differentiation between susceptible and non-susceptible clones and determination of three resistance biomarkers. This approach can be a valuable alternative for the otherwise time-consuming and labor-intensive methods commonly used"
Keywords:		Biomarkers/analysis Chimera Eucalyptus/*chemistry/immunology/metabolism Gas Chromatography-Mass Spectrometry/*methods Multivariate Analysis Plant Diseases/*immunology/microbiology *Plant Immunity Plant Leaves/*chemistry/immunology/metabolism Solid Phase M;
Notes:		"MedlineHantao, Leandro Wang Toledo, Bruna Regina Ribeiro, Fabiana Alves de Lima Pizetta, Marilia Pierozzi, Caroline Geraldi Furtado, Edson Luiz Augusto, Fabio eng Research Support, Non-U.S. Gov't Netherlands 2013/10/24 Talanta. 2013 Nov 15; 116:1079-84. doi: 10.1016/j.talanta.2013.08.033. Epub 2013 Aug 26"