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Sichuan Da Xue Xue Bao Yi Xue Ban

Title:		[Role of Ph-SA in enhancing bactericidal activity of beta-lactam antibiotics]
Author(s):		Tang XY; Zhang J; Guo L; Qiu XQ;
Address:		"Key Laboratory of Transplant Engineering and Immunology, the Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China"
Journal Title:		Sichuan Da Xue Xue Bao Yi Xue Ban
Year:		2006
Volume:		37
Issue:		1
Page Number:		48 - 51
DOI:
ISSN/ISBN:		1672-173X (Print) 1672-173X (Linking)
Abstract:		"OBJECTIVE: To identify the role of Ph-SA in inhibiting beta-lactamase secretion of S. aureus and in enhancing the bactericidal activity of antibiotics against antibiotic resistant bacteria. METHODS: To determine the effects of Ph-SA on beta-lactamase product of bacteria and on bactericidal activity of penicillin, the growth rate and lactamase activity of penicillin-resistant S. aureus cells in exponential growth were detected after treatment with 1.43 X 10(-4) micromol/mL (10 microg/mL) Ph-SA (Group 1), 2.81 x 10(-2) micromol/mL (10 microg/mL) penicillin (Group 2), 0.715 x 10(-4) micromol/mL(5 microg/mL) Ph-SA + 1.40 x 10(-2) micromol/mL (5 microg/mL) penicillin (Group 3) respectively; and a control group was detected for comparison. The same method was used to detect whether Ph-SA could enhance bactericidal activity of Cefazolin. Results It was revealed that 1.43 x 10(-4) micromol/mL (10 microg/mL) Ph-SA could inhibit beta-lactamase activity significantly, whereas no marked bactericidal activity was detected. The growth rate of bacteria cells treated with 0.715 x 10(-4) micromol/mL Ph-SA + 1.40 x 10(-2) mol/mL penicillin and that of bacteria cells treated with 0. 715 x 10(-4) micromol/mL (5 microg/mL) Ph-SA + 1.05 x 10(-2) micromol/mL (5 microg/mL) Cefazolin were the same as those treated by 2.81 x 10(-)2 micromol/mL (10 microg/mL)penicillin and 2.10 x 10(-2) micromol/mL (10 microg/mL) Cefazolin respectively, while the beta-lactamase activities of both groups were lower than control. CONCLUSION: Ph-SA may be of value in enhancing the bactericidal activity of antibiotics and in alleviating the resistance of bacteria by inhibition of beta-lactamase activity"
Keywords:		Anti-Bacterial Agents/*pharmacology Bacterial Proteins/genetics Cefotiam/pharmacology Drug Synergism Microbial Sensitivity Tests Pheromones/genetics Recombinant Fusion Proteins/*pharmacology Staphylococcus aureus/*drug effects/metabolism Trans-Activators/;
Notes:		"MedlineTang, Xiao-yan Zhang, Jie Guo, Li Qiu, Xiao-qing chi English Abstract Research Support, Non-U.S. Gov't China 2006/02/14 Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan; 37(1):48-51"