| Title: | Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production |
| Author(s): | Onda Y; Mochida K; Yoshida T; Sakurai T; Seymour RS; Umekawa Y; Pirintsos SA; Shinozaki K; Ito K; |
| Address: | "1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan. 1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan [3] Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan. Integrated Genome Informatics Research Unit, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan. School of Biological Sciences, University of Adelaide, Australia. United Graduate School of Agricultural Science, Iwate University, Morioka, Japan. Department of Biology and Botanical Garden, University of Crete, Heraklion, Greece. 1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan. Cryobiofrontier Research Center, Iwate University, Morioka, Japan" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-D-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants" |
| Keywords: | "Arum/*genetics/metabolism Biosynthetic Pathways/genetics Energy Metabolism/genetics Flowers/chemistry/*genetics/metabolism Gene Expression Profiling/*methods *Gene Expression Regulation, Plant Gene Ontology Mitochondrial Proteins/genetics/metabolism Odora;" |
| Notes: | "MedlineOnda, Yoshihiko Mochida, Keiichi Yoshida, Takuhiro Sakurai, Tetsuya Seymour, Roger S Umekawa, Yui Pirintsos, Stergios Arg Shinozaki, Kazuo Ito, Kikukatsu eng Research Support, Non-U.S. Gov't England 2015/03/05 Sci Rep. 2015 Mar 4; 5:8753. doi: 10.1038/srep08753" |
