« Previous AbstractA Predictive Model for Yeast Cell Polarization in Pheromone Gradients    Next Abstract[Parameters of the liquid-liquid extraction of toxicologically relevant organic compounds. Part 1: Methodology of the experimental determination of partition constants and pH-dependent partition quotients; results obtained with acidic compounds] »

BMC Genomics

Title:		RNA-seq of eight different poplar clones reveals conserved up-regulation of gene expression in response to insect herbivory
Author(s):		Muller NA; Kersten B; Fladung M; Schroeder H;
Address:		"Thunen Institute of Forest Genetics, Sieker Landstrasse 2, 22927, Grosshansdorf, Germany. Thunen Institute of Forest Genetics, Sieker Landstrasse 2, 22927, Grosshansdorf, Germany. hilke.schroeder@thuenen.de"
Journal Title:		BMC Genomics
Year:		2019
Volume:		20190828
Issue:		1
Page Number:		673 -
DOI:		10.1186/s12864-019-6048-8
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: Herbivorous insects can have a profound impact on plant growth performance. In some years, canopy damage in poplar plantations exceeds 50% of the total leaf surface, thereby possibly compromising carbon fixation and biomass yield. To assess the transcriptional response of elite poplar clones to insect feeding and to test whether this response varies between different genotypes, we performed an RNA-sequencing experiment. We deeply sequenced the transcriptomes of eight elite clones belonging to three poplar species (Populus trichocarpa, P. nigra and P. maximowiczii), under Phratora vitellinae feeding and control conditions. This allowed us to precisely quantify transcript levels of about 24,000 expressed genes. RESULTS: Our data reveal a striking overall up-regulation of gene expression under insect attack in all eight poplar clones studied. The up-regulated genes were markedly enriched for the biological process 'regulation of transcription' indicating a highly concerted restructuring of the transcriptome. A search for potential cis-regulatory elements (CREs) that may be involved in this process identified the G-box (CACGTG) as the most significant motif in the promoters of the induced genes. In line with the role of the G-box in jasmonate (JA)-mediated activation of gene expression by MYC2, several genes involved in JA biosynthesis and signaling were up-regulated in our dataset. A co-expression network analysis additionally highlighted WRKY transcription factors. Within the most prominent expression module, WRKYs were strongly overrepresented and occupied several network hubs. Finally, the insect-induced genes comprised several protein families known to be involved in plant defenses, e.g. cytochrome P450s, chitinases and protease inhibitors. CONCLUSIONS: Our data represent a comprehensive characterization of the transcriptional response of selected elite poplar clones to insect herbivory. Our results suggest that the concerted up-regulation of gene expression is controlled by JA signaling and WRKY transcription factors, and activates several defense mechanisms. Our data highlight potential targets of selection and may thus contribute to breeding insect-resistant poplar clones"
Keywords:		"Animals Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/metabolism Chitinases/metabolism Cyclopentanes/metabolism Cytochrome P-450 Enzyme System/metabolism *Gene Expression Regulation, Plant *Herbivory Insecta/physiology Oxylipins/metabolism P;"
Notes:		"MedlineMuller, Niels A Kersten, Birgit Fladung, Matthias Schroeder, Hilke eng 22000514/Fachagentur Nachwachsende Rohstoffe/ England 2019/08/29 BMC Genomics. 2019 Aug 28; 20(1):673. doi: 10.1186/s12864-019-6048-8"