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Biol Chem Hoppe Seyler

Title:		Qualitative and quantitative determination of pheromone secretion in female gametes of Ectocarpus siliculosus (Phaeophyceae)
Author(s):		Muller DG; Schmid CE;
Address:		Fakultat fur Biologie der Universitat Konstanz
Journal Title:		Biol Chem Hoppe Seyler
Year:		1988
Volume:		369
Issue:		8
Page Number:		647 - 653
DOI:		10.1515/bchm3.1988.369.2.647
ISSN/ISBN:		0177-3593 (Print) 0177-3593 (Linking)
Abstract:		"Pheromone secretion in living female gametes of Ectocarpus siliculosus was examined by closed-loop extraction and gas chromatography. The pheromone (ectocarpene) recovery efficiency was calibrated with synthetic ectocarpene and was found to depend strongly on the volume of the closed-loop system. Maximum extraction efficiency of ectocarpene (17.4%) was obtained with a small-volume system. In addition to the main compound ectocarpene, the pheromone bouquet of E. siliculosus contained minor amounts of hormosirene, multifidene and dictyotene, which until now are known only as sperm-attractants in other brown algae. The pheromone bouquet was identical in 4 clones of different geographic origins. Settled female gametes of Ectocarpus siliculosus continued to secrete pheromone and to attract male gametes for up to 7 h after their release with an ectocarpene secretion rate of 1 x 10(5) molecules s-1 cell-1"
Keywords:		"Chromatography, Gas Cycloheptanes/isolation & purification/*metabolism Eukaryota/*physiology Female Phaeophyta/*physiology Pheromones/isolation & purification/*metabolism Species Specificity Structure-Activity Relationship;"
Notes:		"MedlineMuller, D G Schmid, C E eng Germany 1988/08/01 Biol Chem Hoppe Seyler. 1988 Aug; 369(8):647-53. doi: 10.1515/bchm3.1988.369.2.647"