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Molecules

Title:		Separation of Volatile Metabolites from the Leaf-Derived Essential Oil of Piper mollicomum Kunth (Piperaceae) by High-Speed Countercurrent Chromatography
Author(s):		Marques AM; Peixoto ACC; Provance DW; Kaplan MAC;
Address:		"Laboratorio de Produtos Naturais, PN3, FarManguinhos, FIOCRUZ, 21045-900 Rio de Janeiro, Brazil. andrefarmaciarj@yahoo.com.br. Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, Brazil. acpeixoto1@ufrj.br. Centro de Desenvolvimento Tecnologico em Saude (CDTS/Fiocruz), 21045-360 Rio de Janeiro, Brasil. bill.provance@cdts.fiocruz.br. Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, Brazil. kaplan@nppn.ufrj.br"
Journal Title:		Molecules
Year:		2018
Volume:		20181123
Issue:		12
Page Number:		-
DOI:		10.3390/molecules23123064
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"The technique of high-speed countercurrent chromatography was applied to the isolation of compounds in essential oil derived from the leaves of Piper mollicomum species. Plant leaves (200.0 g) were submitted to hydrodistillation in a modified Clevenger apparatus. The resulting crude leaf essential oil was analyzed by gas chromatography with flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) to determine the profile of the components. The purified fractions were composed of monoterpenes and sesquiterpenes such as camphor (85.0 mg at 98.5% purity), (E)-nerolidol (100.0 mg at 92.8% purity), and camphene (150.0 mg at 82.0% purity). A minor component of the essential oil, bornyl acetate (16.2 mg at 91.2% purity) was also isolated in the one-step separation protocol in 2 h. The countercurrent chromatography technique proved to be a fast and efficient method for the separation of volatile metabolites that conserved the solvent while delivering various fractions of high purity"
Keywords:		"*Chromatography/methods Gas Chromatography-Mass Spectrometry Magnetic Resonance Spectroscopy *Metabolomics/methods Molecular Structure Oils, Volatile/*chemistry Piper/*chemistry Plant Leaves/*chemistry Solvents Volatile Organic Compounds/*chemistry Ccc ca;"
Notes:		"MedlineMarques, Andre M Peixoto, Ana Clarissa C Provance, D William Jr Kaplan, Maria Auxiliadora C eng Switzerland 2018/11/28 Molecules. 2018 Nov 23; 23(12):3064. doi: 10.3390/molecules23123064"