| Title: | Quantification of Oxidized and Unsaturated Bile Alcohols in Sea Lamprey Tissues by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| Author(s): | Li K; Scott AM; Chung-Davidson YW; Bussy U; Patel T; Middleton ZE; Li W; |
| Address: | "Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. like4@msu.edu. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. scottan7@msu.edu. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. chungyuw@msu.edu. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. bussy@msu.edu. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. pateltrinkal@gmail.com. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. middle73@msu.edu. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. liweim@msu.edu" |
| DOI: | 10.3390/molecules21091119 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "A sensitive and reliable method was developed and validated for the determination of unsaturated bile alcohols in sea lamprey tissues using liquid-liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The liver, kidney, and intestine samples were extracted with acetonitrile and defatted by n-hexane. Gradient UHPLC separation was performed using an Acquity BEH C18 column with a mobile phase of water and methanol containing 20 mM triethylamine. Multiple reaction monitoring modes of precursor-product ion transitions for each analyte was used. This method displayed good linearity, with correlation coefficients greater than 0.99, and was validated. Precision and accuracy (RSD %) were in the range of 0.31%-5.28%, while mean recoveries were between 84.3%-96.3%. With this technique, sea lamprey tissue samples were analyzed for unsaturated bile alcohol analytes. This method is practical and particularly suitable for widespread putative pheromone residue analysis" |
| Keywords: | "Animals Cholestanols/*chemistry/*metabolism Chromatography, High Pressure Liquid Lampreys/*metabolism Mass Spectrometry Oxidation-Reduction Uplc-ms/ms fish metabolites sulfated bile alcohol;" |
| Notes: | "MedlineLi, Ke Scott, Anne M Chung-Davidson, Yu-Wen Bussy, Ugo Patel, Trinkal Middleton, Zoe E Li, Weiming eng Switzerland 2016/08/27 Molecules. 2016 Aug 24; 21(9):1119. doi: 10.3390/molecules21091119" |
