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Insect Biochem Mol Biol

Title:		A reference gene set for chemosensory receptor genes of Manduca sexta
Author(s):		Koenig C; Hirsh A; Bucks S; Klinner C; Vogel H; Shukla A; Mansfield JH; Morton B; Hansson BS; Grosse-Wilde E;
Address:		"Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: ckoenig@ice.mpg.de. Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. Electronic address: ariana.hirsh@mssm.edu. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: sbucks@ice.mpg.de. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: cklinner@ice.mpg.de. Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: hvogel@ice.mpg.de. Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur Boston, MA 02115, USA. Electronic address: ashukla@fas.harvard.edu. Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. Electronic address: jmansfie@barnard.edu. Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. Electronic address: bmorton@barnard.edu. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: hansson@ice.mpg.de. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745 Jena, Germany. Electronic address: grosse-wilde@ice.mpg.de"
Journal Title:		Insect Biochem Mol Biol
Year:		2015
Volume:		20150911
Issue:
Page Number:		51 - 63
DOI:		10.1016/j.ibmb.2015.09.007
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context, mainly the interaction with its host plants. In recent years transcriptomic data has led to the discovery of members of all major chemosensory receptor families in the species, but the data was fragmentary and incomplete. Here we present the analysis of the newly available high-quality genome data for the species, supplemented by additional transcriptome data to generate a high quality reference gene set for the three major chemosensory receptor gene families, the gustatory (GR), olfactory (OR) and antennal ionotropic receptors (IR). Coupled with gene expression analysis our approach allows association of specific receptor types and behaviors, like pheromone and host detection. The dataset will provide valuable support for future analysis of these essential chemosensory modalities in this species and in Lepidoptera in general"
Keywords:		"Animals Arthropod Antennae/metabolism Female Gene Expression Profiling Genome, Insect Male Manduca/*genetics/metabolism Multigene Family Phylogeny Receptors, Ionotropic Glutamate/genetics/metabolism Receptors, Odorant/*genetics/metabolism Receptors, Phero;"
Notes:		"MedlineKoenig, Christopher Hirsh, Ariana Bucks, Sascha Klinner, Christian Vogel, Heiko Shukla, Aditi Mansfield, Jennifer H Morton, Brian Hansson, Bill S Grosse-Wilde, Ewald eng GM041247/GM/NIGMS NIH HHS/ Howard Hughes Medical Institute/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2015/09/15 Insect Biochem Mol Biol. 2015 Nov; 66:51-63. doi: 10.1016/j.ibmb.2015.09.007. Epub 2015 Sep 11"