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Analyst

Title:		In situ lipid profiling of insect pheromone glands by direct analysis in real time mass spectrometry
Author(s):		Cetraro N; Yew JY;
Address:		"Pacific Biosciences Research Center, School of Environment and Ocean Science Technology, University of Hawai'i at Manoa, 1993 East West Road, Honolulu, HI 96822, USA. jyew@hawaii.edu. Molecular Bioscience and Bio-Engineering, College of Tropical Agriculture and Human Resources, University of Hawai'i at Manoa, 1955 East West Road, Honolulu, HI 96822"
Journal Title:		Analyst
Year:		2022
Volume:		20220712
Issue:		14
Page Number:		3276 - 3284
DOI:		10.1039/d2an00840h
ISSN/ISBN:		1364-5528 (Electronic) 0003-2654 (Print) 0003-2654 (Linking)
Abstract:		"Lipid pheromones play a significant role in the behavior and ecology of many insects. The characterization of pheromone structures is a significant challenge due to their low abundance and ephemeral nature. Here we present a method for the analysis of lipid molecules from single pheromone glands of Drosophila melanogaster (fruit fly) using Direct Analysis in Real Time mass spectrometry (DART MS). Our results reveal that DART MS analysis of single tissues generates reproducible, species-specific lipid profiles comprised of fatty acids, wax esters, diacylglycerides and triacylglycerides. In addition, the ion source temperature and application of a solvent wash can cause significant qualitative and quantitative changes in the mass spectral profile. Lastly, we show that untargeted chemical fingerprinting of the gland can be used to accurately categorize species according to phylogenetic subgroup or genotype. Collectively, our findings indicate that DART MS is a rapid and powerful method for characterizing a broad range of lipids in tissues with minimal preparation. The application of direct tissue DART MS will expand the 'secretome' of molecules produced by pheromone glands. In addition to its direct relevance to chemical ecology, the method could potentially be used in pharmaceutical studies for the screening and detection of tissue-specific drug metabolites"
Keywords:		Animals Drosophila *Drosophila melanogaster/metabolism *Insecta/metabolism Lipids Mass Spectrometry/methods Pheromones/metabolism Phylogeny;
Notes:		"MedlineCetraro, Nicolas Yew, Joanne Y eng P20 GM125508/GM/NIGMS NIH HHS/ England 2022/06/18 Analyst. 2022 Jul 12; 147(14):3276-3284. doi: 10.1039/d2an00840h"