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Bioresour Technol

Title:		Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia: The consistency of the process and the microbial communities involved
Author(s):		Cerda A; Mejias L; Gea T; Sanchez A;
Address:		"GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: antoni.sanchez@uab.cat"
Journal Title:		Bioresour Technol
Year:		2017
Volume:		20170716
Issue:
Page Number:		1059 - 1068
DOI:		10.1016/j.biortech.2017.07.076
ISSN/ISBN:		1873-2976 (Electronic) 0960-8524 (Linking)
Abstract:		"Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3.1+/-0.5FPUg(-1)DM and 48+/-4Ug(-1)DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2.6.10(-3)kg volatile organic compounds per Mg of coffee husk and negligible NH(3), CH(4) and N(2)O emissions"
Keywords:		*Cellulase *Coffee *Fermentation Reproducibility of Results Cellulase Coffee husk Solid-state fermentation Specialized inoculum Xylanase;
Notes:		"MedlineCerda, Alejandra Mejias, Laura Gea, Teresa Sanchez, Antoni eng England 2017/08/03 Bioresour Technol. 2017 Nov; 243:1059-1068. doi: 10.1016/j.biortech.2017.07.076. Epub 2017 Jul 16"