| Title: | Isoprene emission aids recovery of photosynthetic performance in transgenic Nicotiana tabacum following high intensity acute UV-B exposure |
| Author(s): | Centritto M; Haworth M; Marino G; Pallozzi E; Tsonev T; Velikova V; Nogues I; Loreto F; |
| Address: | "Trees and Timber Institute, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, FI, Italy. Electronic address: mauro.centritto@cnr.it. Institute of Plant Protection, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, FI, Italy. Institute of Plant Protection, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, FI, Italy; Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche, IS, Italy. Institute of AgroEnvironmental and Forest Biology, National Research Council, Via Salaria km 29.300, 00015 Monterotondo Scalo, RM, Italy; Department of Forest Environment and Resources, University of Tuscia, Via San Camillo de Lellis, 01100 Viterbo, Italy. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria. Institute of AgroEnvironmental and Forest Biology, National Research Council, Via Salaria km 29.300, 00015 Monterotondo Scalo, RM, Italy" |
| DOI: | 10.1016/j.plantsci.2014.06.004 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "Isoprene emission by terrestrial plants is believed to play a role in mitigating the effects of abiotic stress on photosynthesis. Ultraviolet-B light (UV-B) induces damage to the photosynthetic apparatus of plants, but the role of isoprene in UV-B tolerance is poorly understood. To investigate this putative protective role, we exposed non-emitting (NE) control and transgenic isoprene emitting (IE) Nicotiana tabacum (tobacco) plants to high intensity UV-B exposure. Methanol emissions increased with UV-B intensity, indicating oxidative damage. However, isoprene emission was unaffected during exposure to UV-B radiation, but declined in the 48 h following UV-B treatment at the highest UV-B intensities of 9 and 15 Wm(-2). Photosynthesis and the performance of photosystem II (PSII) declined to similar extents in IE and NE plants following UV-B exposure, suggesting that isoprene emission did not ameliorate the immediate impact of UV-B on photosynthesis. However, after the stress, photosynthesis and PSII recovered in IE plants, which maintained isoprene formation, but not in NE plants. Recovery of IE plants was also associated with elevated antioxidant levels and cycling; suggesting that both isoprene formation and antioxidant systems contributed to reinstating the integrity and functionality of cellular membranes and photosynthesis following exposure to excessive levels of UV-B radiation" |
| Keywords: | "Butadienes Hemiterpenes/*metabolism Pentanes Photosynthesis Plants, Genetically Modified/physiology/radiation effects Tobacco/*physiology/*radiation effects Ultraviolet Rays Volatile Organic Compounds Antioxidant defense Fluorescence Isoprene Methanol UV-;" |
| Notes: | "MedlineCentritto, Mauro Haworth, Matthew Marino, Giovanni Pallozzi, Emanuele Tsonev, Tsonko Velikova, Violeta Nogues, Isabel Loreto, Francesco eng Research Support, Non-U.S. Gov't Ireland 2014/08/13 Plant Sci. 2014 Sep; 226:82-91. doi: 10.1016/j.plantsci.2014.06.004. Epub 2014 Jun 19" |
