| Title: | Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes |
| Author(s): | Jiang L; Dumlao MC; Donald WA; Steel CC; Schmidtke LM; |
| Address: | "Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide (Waite Campus), Urrbrae, SA 5064, Australia. School of Agricultural, Environmental and Veterinary Science, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. School of Chemistry, Faculty of Science, University of New South Wales, Sydney, NSW 2052, Australia" |
| DOI: | 10.3390/molecules28135227 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Fungal infection of grape berries (Vitis vinifera) by Botrytis cinerea frequently coincides with harvest, impacting both the yield and quality of grape and wine products. A rapid and non-destructive method for identifying B. cinerea infection in grapes at an early stage prior to harvest is critical to manage loss. In this study, zeolitic imidazolate framework-8 (ZIF-8) crystal was applied as an absorbent material for volatile extraction from B. cinerea infected and healthy grapes in a vineyard, followed by thermal desorption gas chromatography-mass spectrometry. The performance of ZIF-8 in regard to absorbing and trapping the targeted volatiles was evaluated with a standard solution of compounds and with a whole bunch of grapes enclosed in a glass container to maintain standard sampling conditions. The results from the sampling methods were then correlated to B. cinerea infection in grapes, as measured and determined by genus-specific antigen quantification. Trace levels of targeted compounds reported as markers of grape B. cinerea infection were successfully detected with in-field sampling. The peak area counts for volatiles 3-octanone, 1-octen-3-one, 3-octanol, and 1-octen-3-ol extracted using ZIF-8 were significantly higher than values achieved using Tenax((R))-TA from field testing and demonstrated good correlation with B. cinerea infection severities determined by B. cinerea antigen detection" |
| Keywords: | *Vitis/chemistry *Wine/analysis Botrytis Gas Chromatography-Mass Spectrometry/methods Fruit/chemistry VOCs analysis metal-organic frameworks plant disease quality assessment rapid sampling thermal desorption GC-MS; |
| Notes: | "MedlineJiang, Liang Dumlao, Morphy C Donald, William A Steel, Christopher C Schmidtke, Leigh M eng IC170100008/Australian Research Council/ Switzerland 2023/07/14 Molecules. 2023 Jul 5; 28(13):5227. doi: 10.3390/molecules28135227" |
