| Title: | Protein that makes sense in the Argentine ant |
| Author(s): | Ishida Y; Chiang V; Leal WS; |
| Address: | "Honorary Maeda-Duffey Laboratory, Department of Entomology, University of California, Davis, CA 95616, USA" |
| DOI: | 10.1007/s00114-002-0368-1 |
| ISSN/ISBN: | 0028-1042 (Print) 0028-1042 (Linking) |
| Abstract: | "With a protein-based approach, we have identified and cloned the cDNA encoding a chemosensory protein (LhumCSP) in the Argentine ant, Linepithema humile. The open reading frame of the cloned cDNA encoded a signal peptide (20 residues), and a mature protein (pI 4.62) of 106 amino acid residues. The calculated molecular mass (12,453 Da) was in agreement with the molecular mass measured by on-line chromatography-electrospray ionization mass spectrometry (12,448 Da), given the formation of two disulfide bridges. LhumCSP shared sequence similarity with various CSPs, particularly those identified and/or cloned from moth species. Also, LhumCSP showed the hallmark of the chemosensory proteins, i.e., four well conserved cysteine residues. The antennal protein was not detected in non-olfactory tissues (leg and thorax) contrary to a putative pheromone-binding protein isolated from the thorax of the red imported fire ant, Solenopsis invicta. In addition, these findings suggest that, as in Orthopterans and Phasmids, the protein that makes sense in the Argentine ant is not an odorant-binding protein, but rather a chemosensory protein" |
| Keywords: | "Amino Acid Sequence Animals Ants/genetics/*physiology Argentina Base Sequence Chemoreceptor Cells/*physiology Cloning, Molecular DNA, Complementary Insect Proteins/genetics/*physiology Molecular Sequence Data Recombinant Proteins/metabolism;" |
| Notes: | "MedlineIshida, Yuko Chiang, Vicky Leal, Walter S eng Germany 2002/11/27 Naturwissenschaften. 2002 Nov; 89(11):505-7. doi: 10.1007/s00114-002-0368-1. Epub 2002 Oct 2" |
