| Title: | Stereospecific linalool production utilizing two-phase cultivation system in Pantoea ananatis |
| Author(s): | Hoshino Y; Moriya M; Matsudaira A; Katashkina JI; Nitta N; Nishio Y; Usuda Y; |
| Address: | "Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Electronic address: yasushi_hoshino@ajinomoto.com. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Electronic address: mika_moriya@ajinomoto.com. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Electronic address: akiko_matsudaira@ajinomoto.com. Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation. Electronic address: Joanna_Katashkina@agri.ru. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Electronic address: nobuhisa_nitta@ajinomoto.com. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan. Electronic address: yousuke_nishio@ajinomoto.com" |
| DOI: | 10.1016/j.jbiotec.2020.09.021 |
| ISSN/ISBN: | 1873-4863 (Electronic) 0168-1656 (Linking) |
| Abstract: | "Linalool is a monoterpene alcohol, which imparts floral scents to a variety of plants and is extensively used in various kinds of products, such as processed foods and beverages for fragrances and flavors. However, linalool from natural resources is racemate, and production of linalool enantiomers is difficult. To produce stereospecific linalool, we evaluated linalool synthase genes (LINS) from plants, such as Actinidia arguta (AaLINS) and Coriandrum sativum (CsLINS) for (S)-specific LINS and a gram-positive bacterium Streptomyces clavuligerus (ScLINS) for (R)-specific LINS, with Pantoea ananatis strain as the host. Among the 16 LINS examined, AaLINS and ScLINS showed the best (S)-linalool production and (R)-linalool production, respectively, with 100 % enantio excess. Co-expression of the mutated farnesyl diphosphate synthase gene, ispA* (S80?ª+F), from Escherichia coli along with the LINS genes also improved linalool production. In order to prevent volatilization and cell toxicity of linalool, two-phase cultivation with isopropyl myristate was done, which had positive effects on linalool production. The carbon flux to the MVA pathway from glucose was increased by inactivating a membrane-bound glucose dehydrogenase. Overall, 5.60?ª+g/L (S)-linalool and 3.71?ª+g/L (R)-linalool were produced from 60.0?ª+g/L glucose by introduction of AaLINS-ispA* and ScLINS-ispA* in P. ananatis, respectively" |
| Keywords: | Acyclic Monoterpenes *Pantoea/genetics Streptomyces Isopropyl myristate Linalool Pantoea ananatis Stereospecificity Two-phase cultivation; |
| Notes: | "MedlineHoshino, Yasushi Moriya, Mika Matsudaira, Akiko Katashkina, Joanna I Nitta, Nobuhisa Nishio, Yousuke Usuda, Yoshihiro eng Netherlands 2020/09/28 J Biotechnol. 2020 Dec 20; 324:21-27. doi: 10.1016/j.jbiotec.2020.09.021. Epub 2020 Sep 24" |
