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Anesthesiology

Title:		Volatile organic compounds during inflammation and sepsis in rats: a potential breath test using ion-mobility spectrometry
Author(s):		Fink T; Wolf A; Maurer F; Albrecht FW; Heim N; Wolf B; Hauschild AC; Bodeker B; Baumbach JI; Volk T; Sessler DI; Kreuer S;
Address:		"From the Department of Anesthesiology, Intensive Care, and Pain Therapy, Saarland University Medical Center, Homburg (Saar), Germany (T.F., A.W., F.M., F.W.A., N.H., B.W., T.V., S.K.); Computational Systems Biology Group, Max Planck Institute for Informatics, Saarbrucken, Germany (A.C.H.); B&S Analytik, BioMedicalCenter, Dortmund, Germany (B.B., J.I.B.); Faculty of Applied Chemistry, Reutlingen University, Reutlingen, Germany (J.I.B.); and Department of Outcomes Research, Cleveland Clinic, Cleveland, Ohio (D.I.S.)"
Journal Title:		Anesthesiology
Year:		2015
Volume:		122
Issue:		1
Page Number:		117 - 126
DOI:		10.1097/ALN.0000000000000420
ISSN/ISBN:		1528-1175 (Electronic) 0003-3022 (Linking)
Abstract:		"BACKGROUND: Multicapillary column ion-mobility spectrometry (MCC-IMS) may identify volatile components in exhaled gas. The authors therefore used MCC-IMS to evaluate exhaled gas in a rat model of sepsis, inflammation, and hemorrhagic shock. METHODS: Male Sprague-Dawley rats were anesthetized and ventilated via tracheostomy for 10 h or until death. Sepsis was induced by cecal ligation and incision in 10 rats; a sham operation was performed in 10 others. In 10 other rats, endotoxemia was induced by intravenous administration of 10 mg/kg lipopolysaccharide. In a final 10 rats, hemorrhagic shock was induced to a mean arterial pressure of 35 +/- 5 mmHg. Exhaled gas was analyzed with MCC-IMS, and volatile compounds were identified using the BS-MCC/IMS-analytes database (Version 1209; B&S Analytik, Dortmund, Germany). RESULTS: All sham animals survived the observation period, whereas mean survival time was 7.9 h in the septic animals, 9.1 h in endotoxemic animals, and 2.5 h in hemorrhagic shock. Volatile compounds showed statistically significant differences in septic and endotoxemic rats compared with sham rats for 3-pentanone and acetone. Endotoxic rats differed significantly from sham for 1-propanol, butanal, acetophenone, 1,2-butandiol, and 2-hexanone. Statistically significant differences were observed between septic and endotoxemic rats for butanal, 3-pentanone, and 2-hexanone. 2-Hexanone differed from all other groups in the rats with shock. CONCLUSIONS: Breath analysis of expired organic compounds differed significantly in septic, inflammation, and sham rats. MCC-IMS of exhaled breath deserves additional study as a noninvasive approach for distinguishing sepsis from inflammation"
Keywords:		"Animals;Animals Breath Tests/*methods Disease Models, Animal Exhalation Inflammation/diagnosis/*metabolism Ions Male Rats Rats, Sprague-Dawley Sepsis/diagnosis/*metabolism Shock, Hemorrhagic/metabolism Spectrum Analysis/*methods Volatile Organic Compounds/*metabo;"
Notes:		"MedlineFink, Tobias Wolf, Alexander Maurer, Felix Albrecht, Frederic W Heim, Nathalie Wolf, Beate Hauschild, Anne C Bodeker, Bertram Baumbach, Jorg I Volk, Thomas Sessler, Daniel I Kreuer, Sascha eng Research Support, Non-U.S. Gov't 2014/08/30 Anesthesiology. 2015 Jan; 122(1):117-26. doi: 10.1097/ALN.0000000000000420"