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Microbiol Res

Title:		Physiological response of Lactuca sativa exposed to 2-nonanone emitted by Bacillus sp. BCT9
Author(s):		Fincheira P; Quiroz A;
Address:		"Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, Chile; Centro de Excelencia en Investigacion Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Av. Francisco Salazar 01145, Temuco, Chile. Centro de Excelencia en Investigacion Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Av. Francisco Salazar 01145, Temuco, Chile; Departamento de Ciencias Quimicas y Recursos Naturales, Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Av. Francisco Salazar 01145, Temuco, Chile. Electronic address: andres.quiroz@ufrontera.cl"
Journal Title:		Microbiol Res
Year:		2019
Volume:		20181103
Issue:
Page Number:		49 - 55
DOI:		10.1016/j.micres.2018.11.002
ISSN/ISBN:		1618-0623 (Electronic) 0944-5013 (Linking)
Abstract:		"Volatile organic compounds (VOCs) released from bacterial species have been reported as plant growth inducers. In this sense, Lactuca sativa was used as model vegetable to prospect the effects of 2-nonanone released by Bacillus sp. BCT9 at cellular and organ structure level, so we present preliminary results about the physiological effects. In this study, 2-day-old L. sativa were exposed to 2-nonanone for 10 days under two delivery systems: 1) 2-nonanone (abrupt delivery) and 2) 2-nonanone + lanolin (controlled delivery). The X-ray elemental microanalysis, scanning electron and confocal laser microscopies techniques were used to evaluate physiological changes 'in vivo' conditions. The results indicated that 2-nonanone increased root and shoot length independently of 2-nonanone delivery system after 7 days of exposition. Additionally, 2-nonanone elicited the increase of anthocyanin and not affects chlorophyll content and electrolyte leakage percentage. The abrupt delivery elicited the increase of both length and density of root hair without causing changes in size of cell epidermis, while controlled delivery induced stomatal opening. Besides, 2-nonanone exposition did not modify the composition and distribution of carbon, nitrogen, phosphorus, potassium, and chlorine in the surface of plant tissue. The results suggested that 2-nonanone acts as a bacterial signal molecule to elicit changes related to root development without damaging the external morphology while epidermal cells at leaf level are not affected, suggesting that 2-nonanone can be an important tool to apply to vegetables"
Keywords:		"Anthocyanins/biosynthesis Bacillus/*metabolism Chlorophyll/metabolism Epidermal Cells/drug effects Ketones/*pharmacology Lanolin/pharmacology Lettuce/*growth & development/microbiology Microscopy, Confocal Microscopy, Electron, Scanning Plant Leaves/metab;"
Notes:		"MedlineFincheira, Paola Quiroz, Andres eng Germany 2019/01/16 Microbiol Res. 2019 Feb; 219:49-55. doi: 10.1016/j.micres.2018.11.002. Epub 2018 Nov 3"