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Proc Natl Acad Sci U S A

Title:		The yeast STE12 protein binds to the DNA sequence mediating pheromone induction
Author(s):		Dolan JW; Kirkman C; Fields S;
Address:		"Department of Microbiology, State University of New York, Stony Brook 11794"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1989
Volume:		86
Issue:		15
Page Number:		5703 - 5707
DOI:		10.1073/pnas.86.15.5703
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"The STE12 gene product of Saccharomyces cerevisiae is required for the transcription of two sets of cell-type-specific genes: the a-specific genes (active only in a cells) and the alpha-specific genes (active only in alpha cells). We show that radiolabeled STE12 protein, prepared by in vitro transcription and translation, is capable of forming complexes with unlabeled DNA fragments from two a-specific genes. Wild-type yeast, but not a ste12 mutant, produce a factor that forms complexes with labeled DNA from these same two genes. We use assays with yeast extracts to localize the binding site for the STE12-dependent activity. This site corresponds to the sequence identified as the pheromone induction element, which is responsible for increased transcription of genes when cells are exposed to alpha-factor or a-factor. Thus the STE12 protein may be an ultimate effector in the signal transduction pathway triggered by pheromone"
Keywords:		"Amino Acid Sequence Base Sequence DNA, Fungal/*genetics/metabolism *Genes *Genes, Fungal Mating Factor Molecular Sequence Data Peptide Biosynthesis Peptides/*genetics Pheromones/*biosynthesis Plasmids Protein Biosynthesis Saccharomyces cerevisiae/*genetic;"
Notes:		"MedlineDolan, J W Kirkman, C Fields, S eng 5 T32 CA09176/CA/NCI NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1989/08/01 Proc Natl Acad Sci U S A. 1989 Aug; 86(15):5703-7. doi: 10.1073/pnas.86.15.5703"