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Proc Natl Acad Sci U S A

Title:		"Vertebrate and yeast calmodulin, despite significant sequence divergence, are functionally interchangeable"
Author(s):		Davis TN; Thorner J;
Address:		"Department of Biochemistry, University of California, Berkeley 94720"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1989
Volume:		86
Issue:		20
Page Number:		7909 - 7913
DOI:		10.1073/pnas.86.20.7909
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Yeast strains relying solely on vertebrate (Xenopus laevis) calmodulin, expressed under control of a yeast (GAL1) promoter, grew at the same rate as yeast cells containing their endogenous calmodulin. Therefore, the ability to perform essential functions has been conserved between yeast and vertebrate calmodulins, suggesting that calmodulin performs the same (or overlapping) roles in yeast as it does in higher eukaryotes. Successful substitution of vertebrate for yeast calmodulin also suggests that the two proteins can adopt similar conformations in vivo, despite the large number of amino acid differences between them (60 out of 148 residues). Strains overproducing either vertebrate or yeast calmodulin about 100-fold and a strain producing a normal level of yeast calmodulin were essentially indistinguishable in many characteristics, including microtubule distribution, rate of secretion, response to mating pheromone, sporulation, and adaptation to nutrient limitation. Calmodulin overproduction did not confer elevated resistance to a phenothiazine drug, trifluoperazine, thought to be a calmodulin-specific inhibitor. These results have important implications for understanding the role of calmodulin in intracellular calcium signaling"
Keywords:		"3', 5'-Cyclic-AMP Phosphodiesterases/metabolism Animals Brain/enzymology Calmodulin/*genetics/isolation & purification/pharmacology Cattle DNA/genetics Gene Expression *Genes Genes, Fungal Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/drug ef;"
Notes:		"MedlineDavis, T N Thorner, J eng CA09041/CA/NCI NIH HHS/ GM21841/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1989/10/01 Proc Natl Acad Sci U S A. 1989 Oct; 86(20):7909-13. doi: 10.1073/pnas.86.20.7909"