| Title: | Sexual pheromone modulates the frequency of cytosolic Ca(2+) bursts in Saccharomyces cerevisiae |
| Author(s): | Carbo N; Tarkowski N; Ipina EP; Dawson SP; Aguilar PS; |
| Address: | "Laboratorio de Biologia Celular de Membranas, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay. Laboratorio de Biologia Celular de Membranas, Instituto de Investigaciones Biotecnologicas, Universidad de San Martin, San Martin 1650CPZ, Argentina. Departamento de Fisica and IFIBA, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina. Laboratorio de Biologia Celular de Membranas, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay paguilar@iib.unsam.edu.ar" |
| ISSN/ISBN: | 1939-4586 (Electronic) 1059-1524 (Print) 1059-1524 (Linking) |
| Abstract: | "Transient and highly regulated elevations of cytosolic Ca(2+) control a variety of cellular processes. Bulk measurements using radioactive Ca(2+) and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells undergo a rise of cytosolic Ca(2+) that is mediated by two import systems composed of the Mid1-Cch1-Ecm7 protein complex and the Fig1 protein. Although this response has been widely studied, there is no treatment of Ca(2+) dynamics at the single-cell level. Here, using protein calcium indicators, we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca(2+) bursts. Most bursts reach maximal amplitude in 1-10 s, range between 7 and 30 s, and decay in a way that fits a single-exponential model. In vegetative cells, bursts are scarce but preferentially occur when cells are transitioning G1 and S phases. On pheromone presence, Ca(2+) burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig1, and a third, unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 undergoes nuclear localization bursts during the pheromone response" |
| Keywords: | Calcium/*metabolism Calcium Channels/metabolism Cytoplasm/metabolism Cytosol/metabolism Membrane Glycoproteins/metabolism Membrane Proteins/metabolism Pheromones/*metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism; |
| Notes: | "MedlineCarbo, Natalia Tarkowski, Nahuel Ipina, Emiliano Perez Dawson, Silvina Ponce Aguilar, Pablo S eng 2016/12/30 Mol Biol Cell. 2017 Feb 15; 28(4):501-510. doi: 10.1091/mbc.E16-07-0481. Epub 2016 Dec 28" |
