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Plasmid

Title:		"The origin of transfer (oriT) of the enterococcal, pheromone-responding, cytolysin plasmid pAD1 is located within the repA determinant"
Author(s):		An FY; Clewell DB;
Address:		"School of Dentistry, The University of Michigan, Ann Arbor, Michigan 48109, USA"
Journal Title:		Plasmid
Year:		1997
Volume:		37
Issue:		1
Page Number:		87 - 94
DOI:		10.1006/plas.1996.1270
ISSN/ISBN:		0147-619X (Print) 0147-619X (Linking)
Abstract:		The highly conjugative pAD1 plasmid encodes a mating response to the peptide sex pheromone cAD1 secreted by recipient strains of Enterococcus faecalis. The location of the origin of transfer (oriT) was determined through the ability to mobilize the Escherichia coli-E. faecalis shuttle plasmid pAM401 carrying specific segments of pAD1. The oriT sequence was found to be completely within the repA determinant known to be required for plasmid maintenance
Keywords:		Bacterial Proteins/genetics Base Sequence Chromosome Mapping *DNA Helicases DNA Primers/genetics *DNA-Binding Proteins Enterococcus faecalis/*drug effects/*genetics Escherichia coli/genetics Molecular Sequence Data Oligopeptides/*pharmacology Plasmids/*ge;
Notes:		"MedlineAn, F Y Clewell, D B eng GM33956/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1997/01/01 Plasmid. 1997; 37(1):87-94. doi: 10.1006/plas.1996.1270"