| Title: | Antagonistic regulation of Fus2p nuclear localization by pheromone signaling and the cell cycle |
| Address: | "Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA" |
| ISSN/ISBN: | 1540-8140 (Electronic) 0021-9525 (Print) 0021-9525 (Linking) |
| Abstract: | "When yeast cells sense mating pheromone, they undergo a characteristic response involving changes in transcription, cell cycle arrest in early G1, and polarization along the pheromone gradient. Cells in G2/M respond to pheromone at the transcriptional level but do not polarize or mate until G1. Fus2p, a key regulator of cell fusion, localizes to the tip of the mating projection during pheromone-induced G1 arrest. Although Fus2p was expressed in G2/M cells after pheromone induction, it accumulated in the nucleus until after cell division. As cells arrested in G1, Fus2p was exported from the nucleus and localized to the nascent tip. Phosphorylation of Fus2p by Fus3p was required for Fus2p export; cyclin/Cdc28p-dependent inhibition of Fus3p during late G1 through S phase was sufficient to block exit. However, during G2/M, when Fus3p was activated by pheromone signaling, Cdc28p activity again blocked Fus2p export. Our results indicate a novel mechanism by which pheromone-induced proteins are regulated during the transition from mitosis to conjugation" |
| Keywords: | "Active Transport, Cell Nucleus/physiology CDC28 Protein Kinase, S cerevisiae/genetics/metabolism *Cell Cycle/drug effects/physiology Cell Cycle Proteins/genetics/metabolism Cell Nucleus/*metabolism Cyclin-Dependent Kinase Inhibitor Proteins Cytoskeletal P;" |
| Notes: | "MedlineYdenberg, Casey A Rose, Mark D eng R01 GM037739/GM/NIGMS NIH HHS/ R01 GM037739-22/GM/NIGMS NIH HHS/ GM37739/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2009/02/04 J Cell Biol. 2009 Feb 9; 184(3):409-22. doi: 10.1083/jcb.200809066. Epub 2009 Feb 2" |
