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Anal Bioanal Chem

Title:		Analysis of volatile organic compounds from deep airway in the lung through intubation sampling
Author(s):		Xu W; Zhang J; Ding H; Ding Y; Zou X; Yang M; Zhou Q; Liu Z; Zheng L; Zuo H; Ge D; Zhang Q; Huang C; Shen C; Chu Y;
Address:		"Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China. University of Science and Technology of China, Hefei, 230026, China. The Second Hospital of Anhui Medical University, Hefei, 230601, China. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China. xzou@cmpt.ac.cn. The Second Hospital of Anhui Medical University, Hefei, 230601, China. yangmin@ahmu.edu.cn. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China. chyshen@aiofm.ac.cn. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, 230031, China. chyshen@aiofm.ac.cn"
Journal Title:		Anal Bioanal Chem
Year:		2022
Volume:		20220826
Issue:		26
Page Number:		7647 - 7658
DOI:		10.1007/s00216-022-04295-x
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Exhaled volatile organic compounds (VOCs) have been widely applied for the study of disease biomarkers. Oral exhalation and nasal exhalation are two of the most common sampling methods. However, VOCs released from food residues and bacteria in the mouth or upper respiratory tract were also sampled and usually mistaken as that produced from body metabolism. In this study, exhalation from deep airway was first directly collected through intubation sampling and analyzed. The exhalation samples of 35 subjects were collected through a catheter, which was inserted into the trachea or bronchus through the mouth and upper respiratory tract. Then, the VOCs in these samples were detected by proton transfer reaction mass spectrometry (PTR-MS). In addition, fast gas chromatography proton transfer reaction mass spectrometry (FGC-PTR-MS) was used to further determine the VOCs with the same mass-to-charge ratios. The results showed that there was methanol, acetonitrile, ethanol, methyl mercaptan, acetone, isoprene, and phenol in the deep airway. Compared with that in oral exhalation, ethanol, methyl mercaptan, and phenol had lower concentrations. In detail, the median concentrations of ethanol, methyl mercaptan, and phenol were 7.3, 0.6, and 23.9 ppbv, while those in the oral exhalation were 80.0, 5.1, and 71.3 ppbv, respectively, which meant the three VOCs mainly originated from the food residues and bacteria in the mouth or upper respiratory tract, rather than body metabolism. The research results in our study can provide references for expiratory VOC research based on oral and nasal exhalation samplings, which are more feasible in clinical practice"
Keywords:		"Humans *Volatile Organic Compounds/analysis Breath Tests/methods Acetone Protons Methanol/analysis Exhalation Lung/chemistry Biomarkers/analysis Ethanol/analysis Acetonitriles Sulfhydryl Compounds/analysis Phenols/analysis Intubation, Intratracheal Deep a;"
Notes:		"MedlineXu, Wei Zhang, Jin Ding, Houwen Ding, Yueting Zou, Xue Yang, Min Zhou, Qiang Liu, Zhou Zheng, Ling Zuo, Heping Ge, Dianlong Zhang, Qiangling Huang, Chaoqun Shen, Chengyin Chu, Yannan eng LHJJ2020006/Joint Fund between the Second Affiliated Hospital of Anhui Medical University and the Center of Medical Physics and Technology of Hefei Institute of Physical Sciences of Chinese Academy of Sciences/ BJPY2021B08/HFIPS Director's Fund/ YZJJ2022QN45/HFIPS Director's Fund/ YZJJZX202009/HFIPS Director's Fund/ 202104d07020003/Anhui Provincial Key R&D Program/ 21705152/National Natural Science Foundation of China/ 21876176/National Natural Science Foundation of China/ 22076190/National Natural Science Foundation of China/ 62171433/National Natural Science Foundation of China/ 2019432/Youth Innovation Promotion Association, CAS, China/ Germany 2022/08/27 Anal Bioanal Chem. 2022 Nov; 414(26):7647-7658. doi: 10.1007/s00216-022-04295-x. Epub 2022 Aug 26"