| Title: | Identification and characterization of aldehyde oxidases (AOXs) in the cotton bollworm |
| Address: | "School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia. W.Xu@Murdoch.edu.au. School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia" |
| DOI: | 10.1007/s00114-017-1515-z |
| ISSN/ISBN: | 1432-1904 (Electronic) 0028-1042 (Linking) |
| Abstract: | "Aldehyde oxidases (AOXs) are a family of metabolic enzymes that oxidize aldehydes into carboxylic acids; therefore, they play critical roles in detoxification and degradation of chemicals. By using transcriptomic and genomic approaches, we successfully identified six putative AOX genes (HarmAOX1-6) from cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). In silico expression profile, reverse transcription (RT)-PCR, and quantitative PCR (qPCR) analyses showed that HarmAOX1 is highly expressed in adult antennae, tarsi, and larval mouthparts, so they may play an important role in degrading plant-derived compounds. HarmAOX2 is highly and specifically expressed in adult antennae, suggesting a candidate pheromone-degrading enzyme (PDE) to inactivate the sex pheromone components (Z)-11-hexadecenal and (Z)-9-hexadecenal. RNA sequencing data further demonstrated that a number of host plants they feed on could significantly upregulate the expression levels of HarmAOX1 in larvae. This study improves our understanding of insect aldehyde oxidases and insect-plant interactions" |
| Keywords: | "Aldehyde Oxidase/*genetics/*metabolism Animals Gene Expression Profiling *Gene Expression Regulation, Enzymologic Larva Moths/*enzymology/*genetics Plants/chemistry Aldehyde oxidase Gene expression Helicoverpa armigera Pheromone-degrading enzyme;" |
| Notes: | "MedlineXu, Wei Liao, Yalin eng Germany 2017/10/25 Naturwissenschaften. 2017 Oct 23; 104(11-12):94. doi: 10.1007/s00114-017-1515-z" |
