| Title: | (E)-beta-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa |
| Author(s): | Wang X; Gao Y; Chen Z; Li J; Huang J; Cao J; Cui M; Ban L; |
| Address: | "Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. College of Animal Science and Technology, China Agricultural University, Beijing, China. The Affiliated High School of Peking University, Beijing, China. National Animal Husbandry Service, Ministry of Agriculture, Beijing, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Pea aphid (Acyrthosiphon pisum Harris) is one of the major pests in alfalfa crops, causing significant yield losses. (E)-beta-farnesene (EbetaF), an alarm pheromone released by pea aphid, is generic to many species of aphids, and is used to minimize potential danger from predators and parasitoids by avoiding the source of the pheromone. RESULTS: In this study, EbetaF synthase gene was constructed into a plant expression vector, and overexpressed in alfalfa (Medicago sativa L.), with expression among transgenic lines confirmed by qRT-PCR. Subcellular localization analysis showed that EbetaF synthase gene was expressed in the plasma membrane and nucleus of the leaf. GC/MS of extraction from transgenic alfalfa indicated emission of EbetaF ranging from 5.92 to 13.09 ng day(-1) g(-1) fresh tissue. Behavior assays in Y-olfactometers demonstrated that transgenic alfalfa expressing AaEbetaF gene could repel pea aphids, with aphids taking a significantly longer time to select a transgenic line compared with the control line (P < 0.01). CONCLUSION: We have demonstrated a potentially valuable strategy of using EbetaF synthase genes for aphid control in alfalfa. (c) 2018 Society of Chemical Industry" |
| Keywords: | "Animals Aphids/*physiology Behavior, Animal Medicago sativa/*genetics/metabolism Pheromones/metabolism Plant Leaves/metabolism Plants, Genetically Modified/*genetics/metabolism Pyrophosphatases/genetics/*metabolism Sesquiterpenes/metabolism (E)-beta-farne;" |
| Notes: | "MedlineWang, Xuemin Gao, Yuhao Chen, Zhihong Li, Jindong Huang, Jianping Cao, Jiangbo Cui, Miaomiao Ban, Liping eng 2014CB138703/National Program on Key Basic Research Project of China/ BAIC09-2018/Beijing Agriculture Innovation Consortium/ 31372364/National Natural Science Foundation of China/ ASTIP-IAS10/Agricultural Science and Technology Innovation Program/ 53CH1/Chinese Academy of Agricultural Sciences/ England 2018/07/28 Pest Manag Sci. 2019 Mar; 75(3):622-631. doi: 10.1002/ps.5153. Epub 2018 Sep 28" |
