« Previous Abstract"A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence"    Next AbstractExtraction and gas chromatography analysis of adult pheromones from the African butterfly Bicyclus anynana »

Proc Natl Acad Sci U S A

Title:		Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae
Author(s):		Brake AJ; Merryweather JP; Coit DG; Heberlein UA; Masiarz FR; Mullenbach GT; Urdea MS; Valenzuela P; Barr PJ;
Address:
Journal Title:		Proc Natl Acad Sci U S A
Year:		1984
Volume:		81
Issue:		15
Page Number:		4642 - 4646
DOI:		10.1073/pnas.81.15.4642
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular/*methods DNA, Recombinant Epidermal Growth Factor/*genetics/metabolism Gene Expression Regulation Genetic Vectors Mating Factor Mutation Peptides/*genetics Plasmids Protein Processing, Post-Translationa;"
Notes:		"MedlineBrake, A J Merryweather, J P Coit, D G Heberlein, U A Masiarz, F R Mullenbach, G T Urdea, M S Valenzuela, P Barr, P J eng 1984/08/01 Proc Natl Acad Sci U S A. 1984 Aug; 81(15):4642-6. doi: 10.1073/pnas.81.15.4642"