« Previous AbstractEngineering yeast for efficient cellulose degradation    Next AbstractFate and transport of monoterpenes through soils. Part I. Prediction of temperature dependent soil fate model input-parameters »

Yeast

Title:		"Nutrient limitation leads to penetrative growth into agar and affects aroma formation in Pichia fabianii, P. kudriavzevii and Saccharomyces cerevisiae"
Author(s):		van Rijswijck IM; Dijksterhuis J; Wolkers-Rooijackers JC; Abee T; Smid EJ;
Address:		"Laboratory of Food Microbiology, Wageningen University, The Netherlands"
Journal Title:		Yeast
Year:		2015
Volume:		20141114
Issue:		1
Page Number:		89 - 101
DOI:		10.1002/yea.3050
ISSN/ISBN:		1097-0061 (Electronic) 0749-503X (Linking)
Abstract:		"Among fermentative yeast species, Saccharomyces cerevisiae is most frequently used as a model organism, although other yeast species may have special features that make them interesting candidates to apply in food-fermentation processes. In this study, we used three yeast species isolated from fermented masau (Ziziphus mauritiana) fruit, S. cerevisiae 131, Pichia fabianii 65 and Pichia kudriavzevii 129, and determined the impact of nitrogen and/or glucose limitation on surface growth mode and the production of volatile organic compounds (VOCs). All three species displayed significant changes in growth mode in all nutrient-limited conditions, signified by the formation of metafilaments or pseudohyphae. The timing of the transition was found to be species-specific. Transition in growth mode is suggested to be linked to the production of certain fusel alcohols, such as phenylethyl alcohol, which serve as quorum-sensing molecules. Interestingly, we did not observe concomitant increased production of phenylethyl alcohol and filamentous growth. Notably, a broader range of esters was found only for the Pichia spp. grown on nitrogen-limited agar for 21 days compared to nutrient-rich agar, and when grown on glucose- and glucose- plus nitrogen-limited agar. Our data suggest that for the Pichia spp., the formation of esters may play an important role in the switch in growth mode upon nitrogen limitation. Further biological or ecological implications of ester formation are discussed"
Keywords:		Culture Media/analysis/*metabolism Esters/analysis/metabolism Fermentation Glucose/analysis/metabolism Hyphae/growth & development/metabolism Nitrogen/analysis/metabolism Pichia/growth & development/*metabolism Saccharomyces cerevisiae/growth & developmen;
Notes:		"Medlinevan Rijswijck, Irma M H Dijksterhuis, Jan Wolkers-Rooijackers, Judith C M Abee, Tjakko Smid, Eddy J eng Research Support, Non-U.S. Gov't England 2014/10/14 Yeast. 2015 Jan; 32(1):89-101. doi: 10.1002/yea.3050. Epub 2014 Nov 14"