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BMC Complement Altern Med

Title:		The effectiveness of traditional Japanese medicine Goshajinkigan in irradiation-induced aspermatogenesis in mice
Author(s):		Takahashi K; Nagahori K; Qu N; Kuramasu M; Hirayanagi Y; Hayashi S; Ogawa Y; Hatayama N; Terayama H; Suyama K; Hirai S; Sakabe K; Itoh M;
Address:		"Department of Anatomy, Tokyo Medical University, Tokyo, 160-8402, Japan. Department of Anatomy, Tokyo Medical University, Tokyo, 160-8402, Japan. quning@tokyo-med.ac.jp. Department of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa, 259-1193, Japan. quning@tokyo-med.ac.jp. Department of Anatomy, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan. Department of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa, 259-1193, Japan"
Journal Title:		BMC Complement Altern Med
Year:		2019
Volume:		20191211
Issue:		1
Page Number:		362 -
DOI:		10.1186/s12906-019-2786-z
ISSN/ISBN:		1472-6882 (Electronic) 1472-6882 (Linking)
Abstract:		"BACKGROUND: Infertility and gonadal dysfunction are well known side-effects by cancer treatment in males. In particularly, chemotherapy and radiotherapy induced testicular damage, resulting in prolonged azoospermia. However, information regarding therapeutics to treat spermatogenesis disturbance after cancer treatment is scarce. Recently, we demonstrated that Goshajinkigan, a traditional Japanese medicine, can completely rescue severe busulfan-induced aspermatogenesis in mice. In this study, we aimed to detect the effects of Goshajinkigan on aspermatogenesis after irradiation. METHODS: This is animal research about the effects of traditional Japanese medicine on infertility after cancer treatment. C57BL/6 J male mice received total body irradiation (TBI: a single dose of 6Gy) at 4 weeks of age and after 60 days were reared a Goshajinkigan (TJ107)-containing or TJ107-free control diet from day 60 to day 120. Then, two untreated females were mated with a single male from each experimental group. On day 60, 120 and 150, respectively, the sets of testes and epididymis of the mice in each group after deep anesthetization were removed for histological and cytological examinations. RESULTS: Histological and histopathological data showed that 6Gy TBI treatment decreased the fertility rate (4/10) in the control diet group; in contrast, in the TJ107-diet group, the fertility rate was 10/10 (p < 0.05 vs. 6Gy group). Supplementation with TJ107 was found to rescue the disrupted inter-Sertoli tight junctions via the normalization of claudin11, occludin, and ZO-1 expression and reduce serum anti-germ cell autoantibodies. CONCLUSIONS: These findings show the therapeutic effect on TBI-induced aspermatogenesis and the recovering disrupted gonadal functions by supplementation with TJ107"
Keywords:		"Animals Azoospermia/*pathology Drugs, Chinese Herbal/*pharmacology Epididymis/cytology/pathology/radiation effects Female Japan Male Medicine, East Asian Traditional Mice Mice, Inbred C57BL Radiation Injuries, Experimental/*pathology Radiation-Protective;"
Notes:		"MedlineTakahashi, Kumpei Nagahori, Kenta Qu, Ning Kuramasu, Miyuki Hirayanagi, Yoshie Hayashi, Shogo Ogawa, Yuki Hatayama, Naoyuki Terayama, Hayato Suyama, Kaori Hirai, Shuichi Sakabe, Kou Itoh, Masahiro eng C: 15K08937; 19K07876/JSPS KAKENHI Grant/ England 2019/12/13 BMC Complement Altern Med. 2019 Dec 11; 19(1):362. doi: 10.1186/s12906-019-2786-z"