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Fungal Genet Biol

Title:		"4-Dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway in Mucor mucedo, is constitutively transcribed but its activity is differently regulated in (+) and (-) mating types"
Author(s):		Schimek C; Petzold A; Schultze K; Wetzel J; Wolschendorf F; Burmester A; Wostemeyer J;
Address:		"Department of General Microbiology and Microbial Genetics, Institute of Microbiology, Friedrich-Schiller-University Jena, Neugasse 24, D-07743 Jena, Germany. b9SCCR@uni-jena.de"
Journal Title:		Fungal Genet Biol
Year:		2005
Volume:		42
Issue:		9
Page Number:		804 - 812
DOI:		10.1016/j.fgb.2005.05.003
ISSN/ISBN:		1087-1845 (Print) 1087-1845 (Linking)
Abstract:		"4-Dihydromethyltrisporate dehydrogenase (TDH) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. In Mucor mucedo, this conversion is required only in the (-) mating type. Expression of the TDH encoding TSP1 gene was analyzed qualitatively using reverse-transcribed PCR. TSP1 is constitutively transcribed in the (+) and in the (-) mating type, irrespective of the mating situation. By immunodetection, the translation product is also formed constitutively. In contrast to gene expression, TDH enzyme activity depends on the sexual status of the mycelium. Activity is restricted to the sexually stimulated (-) mating type. Non-stimulated (-), as well as stimulated and non-stimulated (+) mycelia exhibit no activity and do not influence activity in stimulated (-) mycelia. Time course analysis shows strongly increased enzyme activity at 80 min after stimulation. Low activity exists from the onset of stimulation, indicating that additional regulation mechanisms are involved in TDH function"
Keywords:		"Blotting, Western DNA, Fungal/chemistry/genetics *Gene Expression Regulation, Fungal Molecular Sequence Data Mucor/*enzymology/*genetics Mycelium/enzymology Oxidoreductases/*genetics/*metabolism RNA, Fungal/analysis RNA, Messenger/analysis Reverse Transcr;"
Notes:		"MedlineSchimek, Christine Petzold, Annett Schultze, Kornelia Wetzel, Jana Wolschendorf, Frank Burmester, Anke Wostemeyer, Johannes eng Research Support, Non-U.S. Gov't 2005/07/27 Fungal Genet Biol. 2005 Sep; 42(9):804-12. doi: 10.1016/j.fgb.2005.05.003"