| Title: | Cloning and characterisation of PGA1 and PGA2: two G protein alpha-subunits from pea that promote growth in the yeast Saccharomyces cerevisiae |
| Address: | "Department of Biological Sciences, University of Illinois at Chicago, 60607, USA" |
| DOI: | 10.1046/j.1365-313x.1999.00516.x |
| ISSN/ISBN: | 0960-7412 (Print) 0960-7412 (Linking) |
| Abstract: | "We report here on the cloning and characterization of two G protein alpha-subunits from pea: PGA1 and PGA2. Based on DNA gel blot analysis, PGA1 and PGA2 are the only Galpha homologous sequences in pea. RT-PCR analysis reveals that PGA1 and PGA2 transcripts are present in a variety of adult pea tissues. However, PGA2 mRNA is consistently detected at a lower level than PGA1 and demonstrates some degree of tissue specificity relative to PGA1. In the apical bud of pea seedlings, PGA1 and PGA2 transcripts decrease in response to 24 h of white light following growth for 6 days in darkness. The G protein mediated, yeast mating pathway was used to analyse the function of PGA1 and PGA2 in vivo. PGA1 downregulates the mating pathway, but through a mechanism that is independent of Gbetagamma sequestration. Unexpectedly, both PGA1 and PGA2 promote growth through a mating pathway independent mechanism" |
| Keywords: | "Amino Acid Sequence Base Sequence Cloning, Molecular DNA Primers/genetics Fungal Proteins/genetics GTP-Binding Proteins/chemistry/*genetics Gene Expression Membrane Proteins Molecular Sequence Data Peas/*genetics/growth & development/metabolism Pheromones;" |
| Notes: | "MedlineMarsh, J F 3rd Kaufman, L S eng Research Support, U.S. Gov't, Non-P.H.S. England 1999/09/04 Plant J. 1999 Aug; 19(3):237-47. doi: 10.1046/j.1365-313x.1999.00516.x" |
