« Previous Abstract"Living on the edge: Glucocorticoid physiology in desert iguanas (Dipsosaurus dorsalis) is predicted by distance from an anthropogenic disturbance, body condition, and population density"    Next AbstractAggressive reproductive competition among hopelessly queenless honeybee workers triggered by pheromone signaling »

Biochem Biophys Res Commun

Title:		"Expression of functional soluble forms of human beta-1, 4-galactosyltransferase I, alpha-2,6-sialyltransferase, and alpha-1, 3-fucosyltransferase VI in the methylotrophic yeast Pichia pastoris"
Author(s):		Malissard M; Zeng S; Berger EG;
Address:		"Institute of Physiology, University of Zurich, Winterthurerstrasse 190, Zurich, CH-8057, Switzerland. malissar@physiol.unizh.ch"
Journal Title:		Biochem Biophys Res Commun
Year:		2000
Volume:		267
Issue:		1
Page Number:		169 - 173
DOI:		10.1006/bbrc.1999.1946
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"The cDNAs encoding soluble forms of human beta-1, 4-galactosyltransferase I (EC 2.4.1.22), alpha-2,6-sialyltransferase (EC 2.4.99.1), and alpha-1,3-fucosyltransferase VI (EC 2.4.1.65), respectively, have been expressed in the methylotrophic yeast Pichia pastoris. The vector pPIC9 was used, which contains the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. The recombinant enzymes had similar kinetic properties as their native counterparts. Their identity was confirmed by Western blotting. Recombinant enzymes may be used for in vitro synthesis of oligosaccharides"
Keywords:		"Cloning, Molecular/methods Fucosyltransferases/*genetics/*metabolism Humans Kinetics Lactose Synthase/*genetics/*metabolism Mating Factor Peptides/genetics/physiology Pichia Recombinant Fusion Proteins/genetics Recombinant Proteins/biosynthesis/metabolism;"
Notes:		"MedlineMalissard, M Zeng, S Berger, E G eng Research Support, Non-U.S. Gov't 2000/01/07 Biochem Biophys Res Commun. 2000 Jan 7; 267(1):169-73. doi: 10.1006/bbrc.1999.1946"