| Title: | Insect egg deposition renders plant defence against hatching larvae more effective in a salicylic acid-dependent manner |
| Author(s): | Lortzing V; Oberlander J; Lortzing T; Tohge T; Steppuhn A; Kunze R; Hilker M; |
| Address: | "Institute of Biology, Dahlem Centre of Plant Sciences, Freie Universitat Berlin, Berlin, Germany. Max Planck Institute of Molecular Plant Physiology, Department Secondary Metabolism, Potsdam, Germany" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Plants can improve their antiherbivore defence by taking insect egg deposition as cue of impending feeding damage. Previous studies showed that Pieris brassicae larvae feeding upon egg-deposited Brassicaceae perform worse and gain less weight than larvae on egg-free plants. We investigated how P. brassicae oviposition on Arabidopsis thaliana affects the plant's molecular and chemical responses to larvae. A transcriptome comparison of feeding-damaged leaves without and with prior oviposition revealed about 200 differently expressed genes, including enhanced expression of PR5, which is involved in salicylic acid (SA)-signalling. SA levels were induced by larval feeding to a slightly greater extent in egg-deposited than egg-free plants. The adverse effect of egg-deposited wild-type (WT) plants on larval weight was absent in an egg-deposited PR5-deficient mutant or other mutants impaired in SA-mediated signalling, that is, sid2/ics1, ald1, and pad4. In contrast, the adverse effect of egg-deposited WT plants on larvae was retained in egg-deposited npr1 and wrky70 mutants impaired further downstream in SA-signalling. Oviposition induced accumulation of flavonols in WT plants with and without feeding damage, but not in the PR5-deficient mutant. We demonstrated that egg-mediated improvement of A. thaliana's antiherbivore defence involves SA-signalling in an NPR1-independent manner and is associated with accumulation of flavonols" |
| Keywords: | "Animals Arabidopsis/metabolism/physiology Butterflies/growth & development Gene Expression Profiling Gene Expression Regulation, Plant/physiology *Herbivory Larva/growth & development *Ovum Plant Growth Regulators/*metabolism Plant Leaves/metabolism/physi;" |
| Notes: | "MedlineLortzing, Vivien Oberlander, Jana Lortzing, Tobias Tohge, Takayuki Steppuhn, Anke Kunze, Reinhard Hilker, Monika eng Research Support, Non-U.S. Gov't 2018/09/27 Plant Cell Environ. 2019 Mar; 42(3):1019-1032. doi: 10.1111/pce.13447. Epub 2018 Nov 19" |
