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Insect Biochem Mol Biol

Title:		"CYP345E2, an antenna-specific cytochrome P450 from the mountain pine beetle, Dendroctonus ponderosae Hopkins, catalyses the oxidation of pine host monoterpene volatiles"
Author(s):		Keeling CI; Henderson H; Li M; Dullat HK; Ohnishi T; Bohlmann J;
Address:		"Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4. Electronic address: ckeeling@alumni.sfu.ca"
Journal Title:		Insect Biochem Mol Biol
Year:		2013
Volume:		20131016
Issue:		12
Page Number:		1142 - 1151
DOI:		10.1016/j.ibmb.2013.10.001
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"The mountain pine beetle (MPB, Dendroctonus ponderosae Hopkins) is a significant pest of western North American pine forests. This beetle responds to pheromones and host volatiles in order to mass attack and thus overcome the terpenoid chemical defences of its host. The ability of MPB antennae to rapidly process odorants is necessary to avoid odorant receptor saturation and thus the enzymes responsible for odorant clearance are an important aspect of host colonization. An antenna-specific cytochrome P450, DponCYP345E2, is the most highly expressed transcript in adult MPB antenna. In in vitro assays with recombinant enzyme, DponCYP345E2 used several pine host monoterpenes as substrates, including (+)-(3)-carene, (+)-beta-pinene, (-)-beta-pinene, (+)-limonene, (-)-limonene, (-)-camphene, (+)-alpha-pinene, (-)-alpha-pinene, and terpinolene. The substrates were epoxidized or hydroxylated, depending upon the substrate. To complement DponCYP345E2, we also functionally characterized the NADPH-dependent cytochrome P450 reductase and the cytochrome b5 from MPB. DponCYP345E2 is the first cytochrome P450 to be functionally characterized in insect olfaction and in MPB"
Keywords:		Animals Bicyclic Monoterpenes Bridged Bicyclo Compounds/metabolism Catalysis Coleoptera/metabolism Cyclohexenes/metabolism Cytochrome P-450 Enzyme System/chemistry/*metabolism Host-Parasite Interactions/*genetics Limonene Monoterpenes/metabolism Oxidation;
Notes:		"MedlineKeeling, Christopher I Henderson, Hannah Li, Maria Dullat, Harpreet K Ohnishi, Toshiyuki Bohlmann, Jorg eng Research Support, Non-U.S. Gov't England 2013/10/22 Insect Biochem Mol Biol. 2013 Dec; 43(12):1142-51. doi: 10.1016/j.ibmb.2013.10.001. Epub 2013 Oct 16"