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Mol Cell Biol

Title:		Interactions between the ankyrin repeat-containing protein Akr1p and the pheromone response pathway in Saccharomyces cerevisiae
Author(s):		Kao LR; Peterson J; Ji R; Bender L; Bender A;
Address:		"Department of Biology, Indiana University, Bloomington 47405, USA"
Journal Title:		Mol Cell Biol
Year:		1996
Volume:		16
Issue:		1
Page Number:		168 - 178
DOI:		10.1128/MCB.16.1.168
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Akr1p, which contains six ankyrin repeats, was identified during a screen for mutations that displayed synthetic lethality with a mutant allele of the bud emergence gene BEM1. Cells from which AKR1 had been deleted were alive but misshapen at 30 degrees C and inviable at 37 degrees C. During a screen for mutants that required one or more copies of wild-type AKR1 for survival at 30 degrees C, we isolated mutations in GPA1, which encodes the G alpha subunit of the pheromone receptor-coupled G protein. (The active subunit of this G protein is G beta gamma, and G alpha plays an inhibitory role in G beta gamma-mediated signal transduction.) AKR1 could serve as a multicopy suppressor of the lethality caused by either loss of GPA1 or overexpression of STE4, which encodes the G beta subunit of this G protein, suggesting that pheromone signaling is inhibited by overexpression of Akr1p. Mutations in AKR1 displayed synthetic lethality with a weak allele of GPA1 and led to increased expression of the pheromone-inducible gene FUS1, suggesting that Akr1p normally (and not just when overexpressed) inhibits signaling. In contrast, deletion of BEM1 resulted in decreased expression of FUS1, suggesting that Bem1p normally facilitates pheromone signaling. During a screen for proteins that displayed two-hybrid interactions with Akr1p, we identified Ste4p, raising the possibility that an interaction between Akr1p and Ste4p contributes to proper regulation of the pheromone response pathway"
Keywords:		"Amino Acid Sequence Ankyrins/*genetics Base Sequence DNA Primers/genetics DNA, Fungal/genetics Fungal Proteins/genetics *GTP-Binding Protein alpha Subunits GTP-Binding Protein alpha Subunits, Gq-G11 *GTP-Binding Protein beta Subunits GTP-Binding Proteins/;"
Notes:		"MedlineKao, L R Peterson, J Ji, R Bender, L Bender, A eng GM46271/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1996/01/01 Mol Cell Biol. 1996 Jan; 16(1):168-78. doi: 10.1128/MCB.16.1.168"