| Title: | Methanol utilizers of the rhizosphere and phyllosphere of a common grass and forb host species |
| Author(s): | Kanukollu S; Remus R; Rucker AM; Buchen-Tschiskale C; Hoffmann M; Kolb S; |
| Address: | "Microbial Biogeochemistry, RA1 Landscape Functioning, ZALF Leibniz Centre for Agricultural Landscape Research, Muncheberg, Germany. saranya.kanukollu@zalf.de. Isotope Biogeochemistry and Gas Fluxes, RA1 Landscape Functioning, ZALF Leibniz Centre for Agricultural Landscape Research, Muncheberg, Germany. Max Planck Institute for Biogeochemistry, Hans-Knoll-Strasse 10, 07745, Jena, Germany. Johann Heinrich von Thunen-Institut, Institute of Climate-Smart Agriculture, Braunschweig, Germany. Microbial Biogeochemistry, RA1 Landscape Functioning, ZALF Leibniz Centre for Agricultural Landscape Research, Muncheberg, Germany. steffen.kolb@zalf.de. Thaer Institute, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany. steffen.kolb@zalf.de" |
| DOI: | 10.1186/s40793-022-00428-y |
| ISSN/ISBN: | 2524-6372 (Electronic) 2524-6372 (Linking) |
| Abstract: | "BACKGROUND: Managed grasslands are global sources of atmospheric methanol, which is one of the most abundant volatile organic compounds in the atmosphere and promotes oxidative capacity for tropospheric and stratospheric ozone depletion. The phyllosphere is a favoured habitat of plant-colonizing methanol-utilizing bacteria. These bacteria also occur in the rhizosphere, but their relevance for methanol consumption and ecosystem fluxes is unclear. Methanol utilizers of the plant-associated microbiota are key for the mitigation of methanol emission through consumption. However, information about grassland plant microbiota members, their biodiversity and metabolic traits, and thus key actors in the global methanol budget is largely lacking. RESULTS: We investigated the methanol utilization and consumption potentials of two common plant species (Festuca arundinacea and Taraxacum officinale) in a temperate grassland. The selected grassland exhibited methanol formation. The detection of (13)C derived from (13)C-methanol in 16S rRNA of the plant microbiota by stable isotope probing (SIP) revealed distinct methanol utilizer communities in the phyllosphere, roots and rhizosphere but not between plant host species. The phyllosphere was colonized by members of Gamma- and Betaproteobacteria. In the rhizosphere, (13)C-labelled Bacteria were affiliated with Deltaproteobacteria, Gemmatimonadates, and Verrucomicrobiae. Less-abundant (13)C-labelled Bacteria were affiliated with well-known methylotrophs of Alpha-, Gamma-, and Betaproteobacteria. Additional metagenome analyses of both plants were consistent with the SIP results and revealed Bacteria with methanol dehydrogenases (e.g., MxaF1 and XoxF1-5) of known but also unusual genera (i.e., Methylomirabilis, Methylooceanibacter, Gemmatimonas, Verminephrobacter). (14)C-methanol tracing of alive plant material revealed divergent potential methanol consumption rates in both plant species but similarly high rates in the rhizosphere and phyllosphere. CONCLUSIONS: Our study revealed the rhizosphere as an overlooked hotspot for methanol consumption in temperate grasslands. We further identified unusual new but potentially relevant methanol utilizers besides well-known methylotrophs in the phyllosphere and rhizosphere. We did not observe a plant host-specific methanol utilizer community. Our results suggest that our approach using quantitative SIP and metagenomics may be useful in future field studies to link gross methanol consumption rates with the rhizosphere and phyllosphere microbiome" |
| Keywords: | Metagenome-assembled genomes (MAGs) Methanol Methylotrophs Potential methanol consumption rates Radioactive labelling (14C-methanol) Rhizosphere; |
| Notes: | "PubMed-not-MEDLINEKanukollu, Saranya Remus, Rainer Rucker, Alexander Martin Buchen-Tschiskale, Caroline Hoffmann, Mathias Kolb, Steffen eng KO 2912/9-1/deutsche forschungsgemeinschaft/ England 2022/07/07 Environ Microbiome. 2022 Jul 6; 17(1):35. doi: 10.1186/s40793-022-00428-y" |
