| Title: | Antifungal Activity of Volatile Organic Compounds Produced by Bacillus methylotrophicus and Bacillus thuringiensis against Five Common Spoilage Fungi on Loquats |
| Author(s): | He CN; Ye WQ; Zhu YY; Zhou WW; |
| Address: | "College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China" |
| DOI: | 10.3390/molecules25153360 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Loquat fruit is one of the most perishable fruits in China, and has a very limited shelf life because of mechanical injury and microbial decay. Due to an increasing concern about human health and environmental security, antagonistic microorganisms have been a potential alternative for fungicides to control postharvest diseases. In this work, the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus methylotrophicus BCN2 and Bacillus thuringiensis BCN10 against five postharvest pathogens isolated from loquat fruit, Fusarium oxysporum, Botryosphaeria sp., Trichoderma atroviride, Colletotrichum gloeosporioides, and Penicillium expansum were evaluated by in vitro and in vivo experiments. As a result, the VOCs released by BCN2 and BCN10 were able to suppress the mycelial growth of all targeted pathogens according to inhibition ratio in the double petri-dish assay as well as disease incidence and disease diameter on loquat fruits. The main volatile compounds were identified by solid-phase microextraction (SPME)-gas chromatography. These VOCs produced by the two strains played complementary roles in controlling these five molds and enabled loquat fruits to keep fresh for ten days, significantly. This research will provide a theoretic foundation and technical support for exploring the functional components of VOCs applicable in loquat fruit preservation" |
| Keywords: | "Antifungal Agents/chemistry/*pharmacology Ascomycota/drug effects/growth & development Bacillus/*chemistry Bacillus thuringiensis/*chemistry Chromatography, Gas Colletotrichum/drug effects/growth & development Eriobotrya/*microbiology Hypocreales/drug eff;" |
| Notes: | "MedlineHe, Chao-Nan Ye, Wan-Qiong Zhu, Ying-Ying Zhou, Wen-Wen eng LY20C200005/Natural Science Foundation of Zhejiang Province/ 2018YFA0903200/National Key R & D Program of China/ Switzerland 2020/07/30 Molecules. 2020 Jul 24; 25(15):3360. doi: 10.3390/molecules25153360" |
