| Title: | Detecting volatile compounds from Kraft lignin degradation in the headspace of microbial cultures by selected ion flow tube mass spectrometry (SIFT-MS) |
| Author(s): | Gibson A; Malek L; Dekker RF; Ross B; |
| Address: | "Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada. Electronic address: agibson@lakeheadu.ca. Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada. Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada. Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Division of Medical Sciences, Northern Ontario School of Medicine, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada" |
| DOI: | 10.1016/j.mimet.2015.03.008 |
| ISSN/ISBN: | 1872-8359 (Electronic) 0167-7012 (Linking) |
| Abstract: | "Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was used to quantify methanol and other volatile compounds in the headspace of one bacterial and 12 fungal lignin-degrading microbial cultures. Cultures were grown in 250 mL Erlenmeyer flasks capped with aluminum foil containing 40 mL of nutrient media using Kraft lignin (0.3% w/v) as the sole carbon source. Analysis was done using SIFT-MS with H3O(+) and NO(+) precursors. Product ions were identified with multiple ion mode (MIM). Full scan (FS) mode was used to identify other compounds of interest. Absidia cylindrospora, Ischnoderma resinosum and Pholiota aurivella increased headspace methanol concentration by 136 ppb, 1196 ppb and 278 ppb, respectively, while Flammulina velutipes and Laetiporus sulphureus decreased concentration below ambient levels. F. velutipes and L. sulphureus were found to produce products of methanol oxidation (formaldehyde and formic acid) and were likely metabolizing methanol. Some additional unidentified compounds generated by the fungal cultures are intriguing and will require further study. SIFT-MS can be used to quantify methanol and other volatile compounds in the headspace of microbial cultures and has the potential to be a rapid, sensitive, non-invasive tool useful in elucidating the mechanisms of lignin degradative pathways" |
| Keywords: | Bacteria/growth & development/*metabolism Carbon/metabolism Culture Media/chemistry Fungi/growth & development/*metabolism Lignin/*metabolism Mass Spectrometry/*methods Volatile Organic Compounds/*analysis Kraft lignin Methanol Microbial cultures Sift-ms; |
| Notes: | "MedlineGibson, Andrew Malek, Lada Dekker, Robert F H Ross, Brian eng Research Support, Non-U.S. Gov't Netherlands 2015/03/17 J Microbiol Methods. 2015 May; 112:40-5. doi: 10.1016/j.mimet.2015.03.008. Epub 2015 Mar 12" |
