| Title: | Analysis of volatile emissions from grape berries infected with Aspergillus carbonarius using hyphenated and portable mass spectrometry |
| Author(s): | Giannoukos K; Giannoukos S; Lagogianni C; Tsitsigiannis DI; Taylor S; |
| Address: | "Q Technologies Ltd, 100 Childwall Road, Liverpool, L15 6UX, UK. Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland. stamatios.giannoukos@org.chem.ethz.ch. Laboratory of Plant Pathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens, 118 55, Athens, Greece. Q Technologies Ltd, 100 Childwall Road, Liverpool, L15 6UX, UK. S.Taylor@liv.ac.uk. Mass Spectrometry and Instrumentation Group, Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, L69 3GJ, UK. S.Taylor@liv.ac.uk" |
| DOI: | 10.1038/s41598-020-78332-z |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Mycotoxins represent a serious risk for human and animal health. Omicronchratoxin A (OTA) is a carcinogenic mycotoxin produced by A. carbonarius that constitutes a severe problem for viticulture. In this study, we investigate the development of novel detection and on-line monitoring approaches for the detection of OTA in the field (i.e. out of the chemical laboratory) using advanced molecular sensing. Both stand-alone and hyphenated mass spectrometry (MS) based systems (e.g. Time-of-Flight ToF-MS and gas chromatography GC combined with MS) and compact portable membrane inlet MS (MIMS) have been employed for the first time to detect and monitor volatile emissions of grape berries infected by the fungus Aspergillus carbonarius. In vacuo (electron impact-EI) and ambient ionisation (electrospray ionisation-ESI) techniques were also examined. On-line measurements of the volatile emissions of grape berries, infected by various strains of A. carbonarius with different toxicity levels, were performed resulting in different olfactory chemical profiles with a common core of characteristic mass fragments, which could be eventually used for on-site detection and monitoring allowing consequent improvement in food security" |
| Keywords: | "Aspergillus/*physiology Fruit/*chemistry Gas Chromatography-Mass Spectrometry/*methods Plant Diseases/*microbiology Spectrometry, Mass, Electrospray Ionization Time Factors Vitis/*microbiology Volatile Organic Compounds/*analysis;" |
| Notes: | "MedlineGiannoukos, Konstantinos Giannoukos, Stamatios Lagogianni, Christina Tsitsigiannis, Dimitrios I Taylor, Stephen eng Research Support, Non-U.S. Gov't England 2020/12/05 Sci Rep. 2020 Dec 3; 10(1):21179. doi: 10.1038/s41598-020-78332-z" |
