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J Agric Food Chem

Title:		Development and validation of a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry method for induced changes in Nicotiana attenuata leaves during simulated herbivory
Author(s):		Gaquerel E; Heiling S; Schoettner M; Zurek G; Baldwin IT;
Address:		"Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745 Jena, Germany. egaquerel@ice.mpg.de"
Journal Title:		J Agric Food Chem
Year:		2010
Volume:		58
Issue:		17
Page Number:		9418 - 9427
DOI:		10.1021/jf1017737
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"A liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (HPLC/ESI-TOF-MS) procedure was developed to characterize changes induced in Nicotiana attenuata leaves 1 h and 5 days after wounding and application of Manduca sexta elicitors. The constancy of the measurement conditions was first confirmed for 22 selected analytes spanning the entire chromatogram. Using the Profile Analysis software, we extracted 367 buckets, which were analyzed by principal component analysis and two-factorial ANOVA. One hundred seventy-three buckets were found to be statistically regulated, 128 due to time effects, and 85 due to treatment effects. In vivo 15N-isotope labeling was used to facilitate the annotation and the interpretation of the fragmentation pattern of nitrogen-containing metabolites, and a correlation analysis was performed to test mathematical relationships existing among potential in-source fragments. Additionally, tandem MS measurements of the most regulated ions are presented. Altogether, this study defines a framework for the mining and annotation of major herbivory-elicited changes in Nicotiana attenuata"
Keywords:		"Analysis of Variance Animals Chromatography, High Pressure Liquid/*methods *Manduca Spectrometry, Mass, Electrospray Ionization/*methods Tobacco/*chemistry;"
Notes:		"MedlineGaquerel, Emmanuel Heiling, Sven Schoettner, Matthias Zurek, Gabriela Baldwin, Ian T eng Validation Study 2010/08/13 J Agric Food Chem. 2010 Sep 8; 58(17):9418-27. doi: 10.1021/jf1017737"