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Methods Mol Biol


Title:Chemotropism during yeast mating
Author(s):Follette PJ; Arkowitz RA;
Address:"Institute of Developmental Biology and Cancer, CNRS UMR 6543, University of Nice - Sophia Antipolis, Nice, France"
Journal Title:Methods Mol Biol
Year:2009
Volume:571
Issue:
Page Number:99 - 110
DOI: 10.1007/978-1-60761-198-1_6
ISSN/ISBN:1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:"Virtually all eukaryotic cells can grow in a polarized fashion in response to external signals. Cells can respond to gradients of chemoattractants or chemorepellents by directional growth, a process referred to as chemotropism. The budding yeast Saccharomyces cerevisiae undergoes chemotropic growth during mating, in which two haploid cells of opposite mating type grow toward one another. We have shown that mating pheromone gradients are essential for efficient mating in yeast and have examined the chemotropism defects of different yeast mutants. Two methods of assessing the ability of yeast strains to respond to pheromone gradients are presented here"
Keywords:"Gene Expression Regulation, Fungal Pheromones/metabolism/physiology Saccharomyces cerevisiae/*growth & development/metabolism/*physiology Saccharomyces cerevisiae Proteins/metabolism/physiology Tropism/*physiology;"
Notes:"MedlineFollette, Peter J Arkowitz, Robert A eng Research Support, Non-U.S. Gov't 2009/09/19 Methods Mol Biol. 2009; 571:99-110. doi: 10.1007/978-1-60761-198-1_6"

 
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