| Title: | Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade |
| Author(s): | Flotho A; Simpson DM; Qi M; Elion EA; |
| Address: | "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "Scaffold proteins play pivotal roles during signal transduction. In Saccharomyces cerevisiae, the Ste5p scaffold protein is required for activation of the mating MAPK cascade in response to mating pheromone and assembles a G protein-MAPK cascade complex at the plasma membrane. To serve this function, Ste5p undergoes a regulated localization event involving nuclear shuttling and recruitment to the cell cortex. Here, we show that Ste5p is also subject to two types of phosphorylation and increases in abundance as a result of MAPK activation. During vegetative growth, Ste5p is basally phosphorylated through a process regulated by the CDK Cdc28p. During mating pheromone signaling, Ste5p undergoes increased phosphorylation by the mating MAPK cascade. Multiple kinases of the mating MAPK cascade contribute to pheromone-induced phosphorylation of Ste5p, with the mating MAPKs contributing the most. Pheromone induction or overexpression of the Ste4p Gbeta subunit increases the abundance of Ste5p at a post-translational step, as long as the mating MAPKs are present. Increasing the level of MAPK activation increases the amount of Ste5p at the cell cortex. Analysis of Ste5p localization mutants reveals a strict requirement for Ste5p recruitment to the plasma membrane for the pheromone-induced phosphorylation. These results suggest that the pool of Ste5p that is recruited to the plasma membrane selectively undergoes feedback phosphorylation by the associated MAPKs, leading to an increased pool of Ste5p at the site of polarized growth. These findings provide evidence of a spatially regulated mechanism for post-activation control of a signaling scaffold that potentiates pathway activation" |
| Keywords: | "Adaptor Proteins, Signal Transducing/metabolism/*physiology Binding Sites Blotting, Northern CDC28 Protein Kinase, S cerevisiae/metabolism Cell Membrane/metabolism Enzyme Activation GTP-Binding Protein beta Subunits/metabolism Genes, Reporter Glutathione;" |
| Notes: | "MedlineFlotho, Annette Simpson, David M Qi, Maosong Elion, Elaine A eng R01 GM46962/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2004/08/24 J Biol Chem. 2004 Nov 5; 279(45):47391-401. doi: 10.1074/jbc.M405681200. Epub 2004 Aug 20" |
