| Title: | Combining liquid chromatography and tandem mass spectrometry approaches to the study of monoterpene glycosides (aroma precursors) in wine grape |
| Author(s): | Flamini R; Menicatti M; De Rosso M; Gardiman M; Mayr C; Pallecchi M; Danza G; Bartolucci G; |
| Address: | "Council for Agricultural Research and Economics-Viticulture and Enology (CREA-VE), Viale XXVIII Aprile 26, Conegliano, TV, 31015, Italy. Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, Via U. Schiff 6, 50019 Sesto F.no (FI), Florence, Italy. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNE), University of Padova, Legnaro, PD, Italy. Department of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Viale Morgagni, 50, Florence, FI, 50134, Italy" |
| ISSN/ISBN: | 1096-9888 (Electronic) 1076-5174 (Linking) |
| Abstract: | "Monoterpene-glycosides are important aroma precursors that, undergo hydrolysis, confer intense floral notes to the wines. Therefore, the knowledge of the nature of the sugar residues and the structure of these molecules is of great interest. In present study, liquid chromatography (LC) separation coupled with different mass spectrometry (MS) experiments for the characterization of these compounds were explored. The LC parameters were tuned to optimize the resolution between the analytes present in grape sample extracts. Twenty principal peaks with a relative abundance >1% were selected and divided in 4 classes characterized by different molecular weight. In general, positive ionization of the studied compounds displayed the [M + NH(4) ](+) ion as base peak. On the contrary, a distribution between [M + Cl](-) and [M + HCOO](-) species was observed in negative ion mode. However, a clear differentiation between the studied compounds was only possible by combining both LC and tandem MS (MS/MS). Indeed, by applying a series of energy resolved MS/MS experiments and monitoring both positive and negative ions, a structural characterization of the analytes was achieved. The proposed LC-MS/MS approach provided the profile of monoterpenol-diglycosides and allowed the identification of a series of isobaric terpene-diglycosides in grape. The study of their MS/MS spectra indicated the structure of geranic and/or nerolic acid aglycones. To verify the interest of studied compounds, a preliminary evaluation of the intensity of signals of these glycosides were carried out. The obtained results showed a significant difference between the grape samples collected in two different vintages" |
| Keywords: | "Chromatography, Liquid/*methods Glycosides/*analysis Monoterpenes/*analysis Odorants/*analysis Plant Extracts Tandem Mass Spectrometry/*methods Vitis/*chemistry Wine/analysis Erms Lc-ms/ms aroma glycoside monoterpene wine;" |
| Notes: | "MedlineFlamini, Riccardo Menicatti, Marta De Rosso, Mirko Gardiman, Massimo Mayr, Christine Pallecchi, Marco Danza, Giovanna Bartolucci, Gianluca eng England 2018/06/17 J Mass Spectrom. 2018 Sep; 53(9):792-800. doi: 10.1002/jms.4212. Epub 2018 Jul 9" |
