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Pestic Biochem Physiol

Title:		"Bioactivation of aflatoxin B1 by a cytochrome P450, CYP6AE19 induced by plant signaling methyl jasmonate in Helicoverpa armigra (Hubner)"
Author(s):		Elzaki MEA; Xue RR; Hu L; Wang JD; Zeng RS; Song YY;
Address:		"Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: yyuansong@163.com"
Journal Title:		Pestic Biochem Physiol
Year:		2019
Volume:		20190401
Issue:
Page Number:		211 - 218
DOI:		10.1016/j.pestbp.2019.03.020
ISSN/ISBN:		1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:		"Herbivore attack leads to enhanced production of defensive compounds to mount anti-herbivore defense in plants via activation of the jasmonate signaling pathway. On the other hand, some herbivores can eavesdrop on plants defense signaling and up-regulate their cytochrome P450 genes to increase detoxification of defensive compounds. However, the ecological risk of eavesdropping on plant defense signaling is largely unknown. In this study, we examined the induction of cytochrome P450s by methyl jasmonate (MeJA) and its consequence on the toxicity of aflatoxin B1 (AFB1) to Helicoverpa armigra larvae. The results show that MeJA applications either in a diet or volatile exposure enhanced the toxicity of AFB1 to the larvae. RNA sequences analysis revealed that cytochrome P450 CYP6AE19 was highly induced when MeJA was applied with AFB1. In addition, HaGST encoding glutathione-S-transferase that mainly transforms aflatoxin B1 exo-8,9-epoxide to aflatoxin B1 exo-8,9-glutathione was also induced. RNA interference of CYP6AE19 via injecting a double-stranded RNA decreased mortality of larvae exposed to AFB1; while injecting a double-stranded RNA of HaGST increased larval mortality. Furthermore, a protein model was generated and a subsequent docking simulation for AFB1 suggests the bioactivation as a major mechanism of AFB1. This study provides evidence that MeJA increased larval mortality of H. armigera via induction of CYP6AE19 that can bioactivate AFB1"
Keywords:		Acetates/*pharmacology Aflatoxin B1/*metabolism Animals Cyclopentanes/*pharmacology Cytochrome P-450 Enzyme System/*metabolism Larva/drug effects/metabolism Moths/*drug effects/*metabolism Oxidation-Reduction/drug effects Oxylipins/*pharmacology Aflatoxin;
Notes:		"MedlineElzaki, Mohammed Esmail Abdalla Xue, Rong-Rong Hu, Lin Wang, Jin-da Zeng, Ren-Sen Song, Yuan-Yuan eng 2019/06/04 Pestic Biochem Physiol. 2019 Jun; 157:211-218. doi: 10.1016/j.pestbp.2019.03.020. Epub 2019 Apr 1"