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Curr Genet

Title:		A mutation affecting sexual agglutinability in MATalpha locus of Saccharomyces cerevisiae
Author(s):		Doi S; Yoshimura M;
Address:		"Department of Legal Medicine, Kinki University School of Medicine, Sayama-cho, Minamikawachi-gun, 589, Osaka, Japan"
Journal Title:		Curr Genet
Year:		1985
Volume:		9
Issue:		3
Page Number:		191 - 196
DOI:		10.1007/BF00420311
ISSN/ISBN:		0172-8083 (Print) 0172-8083 (Linking)
Abstract:		"A temperature-sensitive non-agglutinative mutant of Saccharomyces cerevisiae was isolated and characterized. The mutation, sag2, affected sexual agglutination, conjugation and production of alpha-mating pheromone at a restrictive temperature, but not the response to alpha-mating pheromone. Genetic analyses showed that the mutation was recessive and in the MATalpha locus. The sag2 mutation complemented with matalpha2 but not with matalpha1 These results suggest that sag2 is in the MATalpha1 gene and that at a restrictive temperature the mutation, sag2, inactivated the MATalpha1 product, a positive regulator of alpha-mating functions. The sag2 mutation is like matalpha1-5 in its retention of response to alpha-mating pheromone. However, at 25 degrees C, sag2 cells were competent to mate, whereas matalpha1-5 cells were not. Hence, sag2 is regarded as a new allele in the MATalpha1 gene, which we designate matalpha1-11"
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Notes:		"PubMed-not-MEDLINEDoi, S Yoshimura, M eng 1985/03/01 Curr Genet. 1985 Mar; 9(3):191-6. doi: 10.1007/BF00420311"