| Title: | Novel extraction of volatile biomarkers from canine breath for gas chromatography-mass spectrometry |
| Author(s): | Dissanayake S; Lathan P; Mlsna T; |
| Address: | "Department of Chemistry, Mississippi State University, MS 39762, USA" |
| DOI: | 10.1088/1752-7155/6/4/041001 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Here we describe an effective, reproducible, non-invasive volatile organic compound collection and analysis method for exhaled breath gas samples designed specifically for use with dogs. Conditions of the method were optimized, using a range of standard chemicals. This method utilizes a canine mask, two-way non-re-breathing valve, teflon connector, tubing and bag for sample collection. Collection is followed by condensation and headspace solid phase microextraction for sample concentration and gas chromatography-mass spectrometry for analysis. Custom-made glassware, designed to hold the SPME fiber assembly, was cooled to -10 degrees C and used for the collection of the condensate followed by 2 h of headspace extraction at 37 degrees C. Standards show LOD of 0.6-16.8 ppbv, LOQ between 2.1-55.8 ppbv, and good linearity with R(2) between 0.996-0.999 (RSD% 10-19). The method was verified with preliminary results from three dogs demonstrating that this technique is capable of collecting, identifying and quantifying volatile organic chemical constituents in different breath samples" |
| Keywords: | Animals Biomarkers/analysis Breath Tests/methods Dogs *Exhalation Gas Chromatography-Mass Spectrometry/*methods Reproducibility of Results Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineDissanayake, Shamitha Lathan, Patty Mlsna, Todd eng Comparative Study England 2012/09/20 J Breath Res. 2012 Dec; 6(4):041001. doi: 10.1088/1752-7155/6/4/041001. Epub 2012 Sep 18" |
