| Title: | Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis ipsilon Moths |
| Author(s): | Diesner M; Gallot A; Binz H; Gaertner C; Vitecek S; Kahnt J; Schachtner J; Jacquin-Joly E; Gadenne C; |
| Address: | "Department of Biology - Animal Physiology , Philipps University Marburg , D-35032 Marburg , Germany. Institut d'Ecologie et des Sciences de l'Environnement de Paris (UMR iEES-Paris) , INRA , Route de Saint-Cyr , 78026 Versailles Cedex , France. Institute of Zoology , University of Mainz , Johann-Joachim-Becher-Weg 6 , 55128 Mainz , Germany. Max-Planck-Institute fur terrestrische Mikrobiologie, Marburg , Germany. Institut de Genetique, Environnement et Protection des Plantes (UMR IGEPP) , INRA , Agrocampus Ouest, rue Le Notre , 49054 Angers cedex 01 , France" |
| DOI: | 10.1021/acs.jproteome.7b00779 |
| ISSN/ISBN: | 1535-3907 (Electronic) 1535-3893 (Linking) |
| Abstract: | "In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca-corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity" |
| Keywords: | "Animals Central Nervous System/chemistry Chromatography, High Pressure Liquid Female High-Throughput Nucleotide Sequencing Male Moths/*chemistry Neuropeptides/*analysis Peptide Hormones/*analysis Peptides/analysis Proteomics/*methods Sex Factors *Sexual B;" |
| Notes: | "MedlineDiesner, Max Gallot, Aurore Binz, Hellena Gaertner, Cyril Vitecek, Simon Kahnt, Jorg Schachtner, Joachim Jacquin-Joly, Emmanuelle Gadenne, Christophe eng Research Support, Non-U.S. Gov't 2018/02/22 J Proteome Res. 2018 Apr 6; 17(4):1397-1414. doi: 10.1021/acs.jproteome.7b00779. Epub 2018 Feb 28" |
