| Title: | DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli |
| Author(s): | DeLisa MP; Wu CF; Wang L; Valdes JJ; Bentley WE; |
| Address: | "Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park, Maryland 20742, USA" |
| DOI: | 10.1128/JB.183.18.5239-5247.2001 |
| ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
| Abstract: | "Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, a process known as quorum sensing. While the discovery of a diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several quorum sensing genes, the overall number and coordination of genes controlled by quorum sensing through the AI-2 signal has not been studied systematically. We investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesize AI-2. Remarkably, 242 genes, comprising ca. 5.6% of the E. coli genome, exhibited significant transcriptional changes (either induction or repression) in response to a 300-fold AI-2 signaling differential, with many of the identified genes displaying high induction levels (more than fivefold). Significant induction of ygeV, a putative sigma(54)-dependent transcriptional activator, and yhbH, a sigma(54) modulating protein, suggests sigma(54) may be involved in E. coli quorum sensing" |
| Keywords: | "Amino Acid Sequence Bacterial Proteins/genetics/metabolism Culture Media, Conditioned Escherichia coli/*genetics/growth & development/metabolism *Gene Expression Profiling *Gene Expression Regulation, Bacterial Homoserine/analogs & derivatives/genetics/*m;" |
| Notes: | "MedlineDeLisa, M P Wu, C F Wang, L Valdes, J J Bentley, W E eng Research Support, U.S. Gov't, Non-P.H.S. 2001/08/22 J Bacteriol. 2001 Sep; 183(18):5239-47. doi: 10.1128/JB.183.18.5239-5247.2001" |
