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Sci Rep

Title:		Immunomodulatory activity of extracts from five edible basidiomycetes mushrooms in Wistar albino rats
Author(s):		Elhusseiny SM; El-Mahdy TS; Elleboudy NS; Farag MMS; Aboshanab KM; Yassien MA;
Address:		"Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University (ACU), 4th Industrial Area, 6th of October City, Cairo, 2566, Egypt. Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo, 11566, Egypt. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt. Armed Forces College of Medicine (AFCM), Cairo, Egypt. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo, 11566, Egypt. aboshanab2012@pharma.asu.edu.eg"
Journal Title:		Sci Rep
Year:		2022
Volume:		20220720
Issue:		1
Page Number:		12423 -
DOI:		10.1038/s41598-022-16349-2
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Mushrooms are nutritious foods that are widely cultivated all over the world. They are rich in a range of compounds linked to improving functions of the immune system including carotenoids, alkaloids, lectins, enzymes, folates, fats, organic acids, minerals, polysaccharides, phenolics, proteins, tocopherols, terpenoids, and volatile compounds. In this study we investigated, the immunomodulatory activity in rats of the aqueous extracts of five of the most common edible mushrooms belonging to Family Basidiomycota-white-rot fungi including, Lentinula edodes, Agaricus bisporus, Pleurotus ostreatus, Pleurotus columbinus, and Pleurotus sajor-caju. Male Wistar albino rats were assigned to thirteen groups and Immunosuppression was induced by oral administration of dexamethasone (0.1 mg/kg), followed by oral administration of the mushroom extracts at low (200 mg/kg) and high (400 mg/kg) doses. A positive control group received the immune stimulant Echinacea extract Immulant(R) at (30 mg/kg), while the negative control group received only saline. From each animal, in each group, blood samples were collected after 15 days for complete blood counts and for measurement of immunologic parameters, including lysozyme activity, nitric oxide (NO) production and serum cytokines including tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin 1 beta (IL-1beta) levels. Results have shown that white blood cells (WBCs) and lymphocytic counts were significantly boosted by high doses of each of the five mushroom extracts (207-289% increase for WBC and 153-175% for lymphocytes) with a significant increase in lysozyme activity (110-136% increase), NO concentration (159-232% increase) and cytokines as compared to the negative control group. Histopathological examination of the rats' spleen and thymus tissues has shown marked lymphocytic proliferation that was more obvious at the higher doses. In conclusion, our results showed that the five edible mushroom extracts revealed significant immunostimulatory effects preclinically particularly, at the higher doses (400 mg/kg) which can be considered the effective dose"
Keywords:		"*Agaricus Animals Cytokines Echinacea Male *Muramidase Plant Extracts Rats Rats, Wistar;"
Notes:		"MedlineElhusseiny, Shaza M El-Mahdy, Taghrid S Elleboudy, Nooran S Farag, Mohamed M S Aboshanab, Khaled M Yassien, Mahmoud A eng Research Support, Non-U.S. Gov't England 2022/07/21 Sci Rep. 2022 Jul 20; 12(1):12423. doi: 10.1038/s41598-022-16349-2"